In situ probing of intracellular pH by fluorescence from inorganic nanoparticles

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Junhong Guo
  • Shijie Xiong
  • Xinglong Wu
  • Jiancang Shen
  • Paul K. Chu

Detail(s)

Original languageEnglish
Pages (from-to)9183-9189
Journal / PublicationBiomaterials
Volume34
Issue number36
Online published3 Sep 2013
Publication statusPublished - Dec 2013

Abstract

Intracellular pH (pHi) plays a critical role in the physiological processes of cells. Nanoscale sensors based on pH-sensitive fluorescent proteins attached on nanoparticles (NPs) have been designed but inorganic NP-dependent fluorescent nanosensors have not yet been explored. Herein we describe a pH sensitive inorganic semiconductor fluorescent probe based on ultrathin 3C-SiC NPs which can effectively monitor pH in the range of 5.6-7.4 by taking advantage of the linear dependence between the fluorescent intensity ratio of the surface OH- and H+ bonding states to band-to-band recombination and pH. Detection of pHi is demonstrated in living HeLa cells. In particular, pHi measurements during apoptosis confirm the validity and sensitivity of this technique in monitoring real-time changes in the intracellular environment. Toxicity assessment and confocal laser scanning microscopy indicate that the 3C-SiC NPs have low cytotoxicity and are compatible with living cells. © 2013 Elsevier Ltd.

Research Area(s)

  • Intracellular pH detection, Nanoparticles, PH-dependent photoluminescence, Silicon carbide

Citation Format(s)

In situ probing of intracellular pH by fluorescence from inorganic nanoparticles. / Guo, Junhong; Xiong, Shijie; Wu, Xinglong; Shen, Jiancang; Chu, Paul K.

In: Biomaterials, Vol. 34, No. 36, 12.2013, p. 9183-9189.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review