In situ hybridization to detect spatial gene expression in medaka

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalNot applicablepeer-review

7 Scopus Citations
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Author(s)

  • A. R. Tompsett
  • J. W. Park
  • X. Zhang
  • P. D. Jones
  • J. L. Newsted
  • E. X H Chen
  • R. Yu
  • R. S S Wu
  • J. P. Giesy
  • M. Hecker

Detail(s)

Original languageEnglish
Pages (from-to)1257-1264
Journal / PublicationEcotoxicology and Environmental Safety
Volume72
Issue number4
Publication statusPublished - May 2009

Abstract

A whole-animal tissue section in situ hybridization (ISH) system with radio-labeled probes was developed to detect differential gene expression among tissues of the small, oviparous teleost fish, Japanese medaka (Oryzias latipes). Because of its tissue- and gender-specific expression, gonadal aromatase (CYP19a) was selected as a model gene to demonstrate the potential of the system. The ISH system was validated with a 7 d exposure to the model aromatase inhibitor, fadrozole. Fadrozole did not affect the magnitude of gene expression in testes, but significantly up-regulated CYP19a gene expression in ovaries. These results were confirmed with quantitative real-time-polymerase chain reaction (RT-PCR). Histological evaluation revealed that females exposed to 100 μg/L fadrozole lacked mature oocytes. Male gonadal morphology was normal in all treatments. The ISH method developed in this study allowed tissue-specific resolution of gene expression in a whole animal model, as well as the ability to analyze cellular morphological detail in the same organism. © 2008 Elsevier Inc. All rights reserved.

Research Area(s)

  • Aromatase, Autoradiography, CYP19, Endocrine disruption, Fadrozole, Gene expression, Histology, Hormones, Oryzias latipes, RT-PCR

Citation Format(s)

In situ hybridization to detect spatial gene expression in medaka. / Tompsett, A. R.; Park, J. W.; Zhang, X.; Jones, P. D.; Newsted, J. L.; Au, D. W T; Chen, E. X H; Yu, R.; Wu, R. S S; Kong, R. Y C; Giesy, J. P.; Hecker, M.

In: Ecotoxicology and Environmental Safety, Vol. 72, No. 4, 05.2009, p. 1257-1264.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalNot applicablepeer-review