Impact of SARS-coronavirus-encoded proteins on cellular signalling pathways and cytokine/chemokine gene expression
Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal › peer-review
Author(s)
Detail(s)
| Original language | English |
|---|---|
| Pages (from-to) | 16-19 |
| Journal / Publication | Hong Kong medical journal = Xianggang yi xue za zhi / Hong Kong Academy of Medicine |
| Volume | 18 |
| Issue number | 4 (Suppl 3) |
| Publication status | Published - Aug 2012 |
| Externally published | Yes |
Link(s)
| Document Link | Links
|
|---|---|
| Link to Scopus | https://www.scopus.com/record/display.uri?eid=2-s2.0-84873312839&origin=recordpage |
| Permanent Link | https://scholars.cityu.edu.hk/en/publications/publication(262ab64f-9a85-4052-9bc2-c94fbe43d4a2).html |
Abstract
1. Although GFP-tagged ORF8 and ORF3 potently activate the JNK and p38 MAPK pathways, expression of non-tagged SARS-CoV-encoded ORF8 or ORF3 has no obvious effect.
2. Although both GFP-tagged ORF8 and ORF3 induce cell death, expression of non-tagged ORF8 or ORF3 has no obvious effect on cell survival.
3. Addition of an epitope tag to a protein of interest, a common way to study novel proteins in the absence of suitable antibodies, may generate unexpected artefacts. Caution should be taken with any results derived from epitope-tagged proteins.
4. When studying a novel protein, it is essential to prepare suitable antibodies to facilitate detection and purification (eg by immunoprecipitation) of the native or endogenous proteins.
2. Although both GFP-tagged ORF8 and ORF3 induce cell death, expression of non-tagged ORF8 or ORF3 has no obvious effect on cell survival.
3. Addition of an epitope tag to a protein of interest, a common way to study novel proteins in the absence of suitable antibodies, may generate unexpected artefacts. Caution should be taken with any results derived from epitope-tagged proteins.
4. When studying a novel protein, it is essential to prepare suitable antibodies to facilitate detection and purification (eg by immunoprecipitation) of the native or endogenous proteins.
Citation Format(s)
Impact of SARS-coronavirus-encoded proteins on cellular signalling pathways and cytokine/chemokine gene expression. / Chan, J.K.L.; Cheung, P.; He, M. et al.
In: Hong Kong medical journal = Xianggang yi xue za zhi / Hong Kong Academy of Medicine, Vol. 18, No. 4 (Suppl 3), 08.2012, p. 16-19.
In: Hong Kong medical journal = Xianggang yi xue za zhi / Hong Kong Academy of Medicine, Vol. 18, No. 4 (Suppl 3), 08.2012, p. 16-19.
Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal › peer-review
