Abstract
1. Although GFP-tagged ORF8 and ORF3 potently activate the JNK and p38 MAPK pathways, expression of non-tagged SARS-CoV-encoded ORF8 or ORF3 has no obvious effect.
2. Although both GFP-tagged ORF8 and ORF3 induce cell death, expression of non-tagged ORF8 or ORF3 has no obvious effect on cell survival.
3. Addition of an epitope tag to a protein of interest, a common way to study novel proteins in the absence of suitable antibodies, may generate unexpected artefacts. Caution should be taken with any results derived from epitope-tagged proteins.
4. When studying a novel protein, it is essential to prepare suitable antibodies to facilitate detection and purification (eg by immunoprecipitation) of the native or endogenous proteins.
2. Although both GFP-tagged ORF8 and ORF3 induce cell death, expression of non-tagged ORF8 or ORF3 has no obvious effect on cell survival.
3. Addition of an epitope tag to a protein of interest, a common way to study novel proteins in the absence of suitable antibodies, may generate unexpected artefacts. Caution should be taken with any results derived from epitope-tagged proteins.
4. When studying a novel protein, it is essential to prepare suitable antibodies to facilitate detection and purification (eg by immunoprecipitation) of the native or endogenous proteins.
| Original language | English |
|---|---|
| Pages (from-to) | 16-19 |
| Journal | Hong Kong medical journal = Xianggang yi xue za zhi / Hong Kong Academy of Medicine |
| Volume | 18 |
| Issue number | 4 (Suppl 3) |
| Publication status | Published - Aug 2012 |
| Externally published | Yes |
