Immunogenicity of Calvenza-03 EIV/EHV® Vaccine in Horses : Comparative In Vivo Study

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

3 Scopus Citations
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  • Selvaraj Pavulraj
  • Tobias Bergmann
  • Claudia Maria Trombetta
  • Serena Marchi
  • Emanuele Montomoli
  • Sidi Sefiane El Alami
  • Roberto Ragni‐alunni
  • Walid Azab


Original languageEnglish
Article number166
Journal / PublicationVaccines
Issue number2
Online published17 Feb 2021
Publication statusPublished - Feb 2021



Equine influenza (EI) is a highly contagious acute respiratory disease of equines that is caused mainly by the H3N8 subtype of influenza A virus. Vaccinating horses against EI is the most effective strategy to prevent the infection. The current study aimed to compare the kinetics of EI-specific humoral‐ and cell‐mediated immunity (CMI) in horses receiving either identical or mixed vaccinations. Two groups of horses were previously (six months prior) vaccinated with either Calvenza 03 EIV EHV® (G1) or Fluvac Innovator® (G2) vaccine. Subsequently, both groups received a booster single dose of Calvenza 03 EIV EHV® . Immune responses were assessed after 10 weeks using single radial hemolysis (SRH), virus neutralization (VN), and EliSpot assays. Our results revealed that Calvenza‐03 EIV/EHV®‐immunized horses had significantly higher protective EI‐specific SRH antibodies and VN antibodies. Booster immunization with Calvenza‐03 EIV/EHV® vaccine significantly stimulated cell‐mediated immune response as evidenced by significant increase in interferon‐γ‐secreting peripheral blood mononuclear cells. In conclusion, Calvenza‐03 EIV/EHV® vaccine can be safely and effectively used for booster immunization to elicit optimal long persisting humoral and CMI responses even if the horses were previously immunized with a heterogeneous vaccine.

Research Area(s)

  • Equine influenza, Florida clade 2, Horses, Immunogenicity, Vaccination

Citation Format(s)

Immunogenicity of Calvenza-03 EIV/EHV® Vaccine in Horses: Comparative In Vivo Study. / Pavulraj, Selvaraj; Bergmann, Tobias; Trombetta, Claudia Maria et al.
In: Vaccines, Vol. 9, No. 2, 166, 02.2021.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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