Immuno-fluorescence detection of snake venom by using single bead as the assay platform

R. Gao; Y. Zhang; P. Gopalakrishnakone

doi:10.1080/17458080701713953

Author(s)

R. Gao
Y. Zhang
P. Gopalakrishnakone

Detail(s)

Original language	English
Pages (from-to)	111-119
Journal / Publication	Journal of Experimental Nanoscience
Volume	3
Issue number	2
Publication status	Published - Jun 2008
Externally published	Yes

Link(s)

DOI	DOI https://doi.org/10.1080/17458080701713953 Final Published version
	Check@CityULib
Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-47949132010&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(36c32d9c-2401-45e9-9ef3-d84c62a9bf05).html

Abstract

By using micro-scale polystyrene beads as a platform, a rapid and sensitive method for the detection of snake venom was established. In the method, anti-venom antibody or BSA was covalently fixed onto the microsbead to form the capture-bead or control bead. In first step of the experiment, the venom binds to the capture-bead to form the complex through the antibody-antigen interaction. The Qdot conjugated second antibody was then added. The second antibody targeted the Qdot to the capture-bead/antigen complex and form Qdot-second antibody-antigen-capture-bead complex. This complex can be directly observed under UV-microscope. The system was applied to the testing of Naja kaouthia venom and the detection limit of this method was 5-10 ng/ ml.

Research Area(s)

Immunoassay, Qdot, Single bead, Snake venom

Bibliographic Note

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Citation Format(s)

[ RIS ] [ BibTeX ]

Immuno-fluorescence detection of snake venom by using single bead as the assay platform. / Gao, R.; Zhang, Y.; Gopalakrishnakone, P.
In: Journal of Experimental Nanoscience, Vol. 3, No. 2, 06.2008, p. 111-119.

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

Gao, R, Zhang, Y & Gopalakrishnakone, P 2008, 'Immuno-fluorescence detection of snake venom by using single bead as the assay platform', Journal of Experimental Nanoscience, vol. 3, no. 2, pp. 111-119. https://doi.org/10.1080/17458080701713953

Gao, R., Zhang, Y., & Gopalakrishnakone, P. (2008). Immuno-fluorescence detection of snake venom by using single bead as the assay platform. Journal of Experimental Nanoscience, 3(2), 111-119. https://doi.org/10.1080/17458080701713953

Gao R, Zhang Y, Gopalakrishnakone P. Immuno-fluorescence detection of snake venom by using single bead as the assay platform. Journal of Experimental Nanoscience. 2008 Jun;3(2):111-119. doi: 10.1080/17458080701713953

Gao, R. ; Zhang, Y. ; Gopalakrishnakone, P. / Immuno-fluorescence detection of snake venom by using single bead as the assay platform. In: Journal of Experimental Nanoscience. 2008 ; Vol. 3, No. 2. pp. 111-119.