Abstract
By using micro-scale polystyrene beads as a platform, a rapid and sensitive method for the detection of snake venom was established. In the method, anti-venom antibody or BSA was covalently fixed onto the microsbead to form the capture-bead or control bead. In first step of the experiment, the venom binds to the capture-bead to form the complex through the antibody-antigen interaction. The Qdot conjugated second antibody was then added. The second antibody targeted the Qdot to the capture-bead/antigen complex and form Qdot-second antibody-antigen-capture-bead complex. This complex can be directly observed under UV-microscope. The system was applied to the testing of Naja kaouthia venom and the detection limit of this method was 5-10 ng/ ml.
| Original language | English |
|---|---|
| Pages (from-to) | 111-119 |
| Journal | Journal of Experimental Nanoscience |
| Volume | 3 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jun 2008 |
| Externally published | Yes |
Bibliographical note
Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].Research Keywords
- Immunoassay
- Qdot
- Single bead
- Snake venom
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