Immune competence assessment in marine medaka (Oryzias melastigma) : a holistic approach for immunotoxicology

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

8 Scopus Citations
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Author(s)

  • Rui Ye
  • Shin-Ichi Kitamura
  • Jae-Seong Lee
  • Stephen K W Tsui

Detail(s)

Original languageEnglish
Pages (from-to)27687-27701
Journal / PublicationEnvironmental Science and Pollution Research
Volume24
Online published29 Jul 2016
Publication statusPublished - Dec 2017

Abstract

Many anthropogenic pollutants in coastal marine environments can induce immune impairments in wild fish and reduce their survival fitness. There is a pressing need to establish sensitive and high throughput in vivo tools to systematically evaluate the immunosuppressive effects of contaminants in marine teleosts. This study reviewed a battery of in vivo immune function detection technologies established for different biological hierarchies at molecular (immune function pathways and genes by next generation sequencing (NGS)), cellular (leukocytes profiles by flow cytometry), tissues/organ system (whole adult histo-array), and organism (host resistance assays (HRAs)) levels, to assess the immune competence of marine medaka Oryzias melastigma. This approach enables a holistic assessment of fish immune competence under different chemical exposure or environmental scenarios. The data obtained will also be useful to unravel the underlying immunotoxic mechanisms. Intriguingly, NGS analysis of hepatic immune gene expression profiles (male > female) are in support of the bacterial HRA findings, in which infection-induced mortality was consistently higher in females than inmales.As such, reproductive stages and gender-specific responses must be taken into consideration when assessing the risk of immunotoxicants in the aquatic environment. The distinct phenotypic sexual dimorphism and short generation time (3 months) of marine medaka offer additional advantages for sex-related immunotoxicological investigation. 

Research Area(s)

  • Blood leukocytes profiles, Fish immunotoxicology, Hepatic immune genes, Immune system histopathology, Microbial pathogens, O.melastigma, Sexual dimorphism

Citation Format(s)

Immune competence assessment in marine medaka (Oryzias melastigma) : a holistic approach for immunotoxicology. / Ye, Rui; Peterson, Drew Ryan; Seemann, Frauke et al.

In: Environmental Science and Pollution Research, Vol. 24, 12.2017, p. 27687-27701.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review