Immobilization of Recombinant Vault Nanoparticles on Solid Substrates

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

15 Scopus Citations
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Author(s)

  • Yun Xia
  • Yamini Ramgopal
  • Hai Li
  • Lei Shang
  • Parisa Srinivas
  • Valerie A. Kickhoefer
  • Leonard H. Rome
  • Peter R. Preiser
  • Freddy Boey
  • Subbu S. Venkatraman

Detail(s)

Original languageEnglish
Pages (from-to)1417-1424
Journal / PublicationACS Nano
Volume4
Issue number3
Online published10 Feb 2010
Publication statusPublished - 23 Mar 2010
Externally publishedYes

Abstract

Native vaults are nanoscale particles found abundantly in the cytoplasm of most eukaryotic cells. They have a capsule-like structure with a thin shell surrounding a "hollow" interior compartment. Recombinant vault particles were found to self-assemble following expression of the major vault protein (MVP) in a baculovirus expression system, and these particles are virtually identical to native vaults. Such particles have been recently studied as potential delivery vehicles. In this study, we focus on immobilization of vault particles on a solid substrate, such as glass, as a first step to study their interactions with cells. To this end, we first engineered the recombinant vaults by fusing two different tags to the C-terminus of MVP, a 3 amino acid RGD peptide and a 12 amino acid RGD-strep-tag peptide.Wehave demonstrated two strategies for immobilizing vaults on solid substrates. The barrel-and-cap structure of vault particles was observed for the first time, by atomic force microscopy (AFM), in a dry condition. This work proved the feasibility of immobilizing vault nanoparticles on a material surface, and the possibility of using vault nanoparticles as localized and sustainable drug carriers as well as a biocompatible surface moiety. © 2010 American Chemical Society.

Research Area(s)

  • AFM, RGD, self-assembly, strep-tag, TEM, vaults

Citation Format(s)

Immobilization of Recombinant Vault Nanoparticles on Solid Substrates. / Xia, Yun; Ramgopal, Yamini; Li, Hai; Shang, Lei; Srinivas, Parisa; Kickhoefer, Valerie A.; Rome, Leonard H.; Preiser, Peter R.; Boey, Freddy; Zhang, Hua; Venkatraman, Subbu S.

In: ACS Nano, Vol. 4, No. 3, 23.03.2010, p. 1417-1424.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review