Illuminating the dark depths inside coral

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Detail(s)

Original languageEnglish
Article numbere13122
Journal / PublicationCellular Microbiology
Volume22
Issue number1
Online published21 Oct 2019
Publication statusPublished - Jan 2020

Abstract

The ability to observe in situ 3D distribution and dynamics of endosymbionts in corals is crucial for gaining a mechanistic understanding of coral bleaching and reef degradation. Here, we report the development of a tissue clearing (TC) coupled with light sheet fluorescence microscopy (LSFM) method for 3D imaging of the coral holobiont at single-cell resolution. The initial applications have demonstrated the ability of this technique to provide high spatial resolution quantitative information of endosymbiont abundance and distribution within corals. With specific fluorescent probes or assays, TC-LSFM also revealed spatial distribution and dynamics of physiological conditions (such as cell proliferation, apoptosis, and hypoxia response) in both corals and their endosymbionts. This tool is highly promising for in situ and in-depth data acquisition to illuminate coral symbiosis and health conditions in the changing marine environment, providing fundamental information for coral reef conservation and restoration.

Research Area(s)

  • 3D imaging, cellular hypoxia, coral, light sheet fluorescence microscopy, Symbiodiniaceae, tissue clearing, GENE-EXPRESSION, CLIMATE-CHANGE, TEMPORAL PATTERNS, REEF, MICROSCOPY, GENOME, RESILIENCE, MECHANISMS, DIVERSITY, STRESS

Citation Format(s)

Illuminating the dark depths inside coral. / Liu, Chichi; Cheng, Shuk Han; Lin, Senjie.

In: Cellular Microbiology, Vol. 22, No. 1, e13122, 01.2020.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review