Identification of Glycopeptides with Multiple Hydroxylysine O-Glycosylation Sites by Tandem Mass Spectrometry

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

5 Scopus Citations
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Author(s)

  • Yanlin Zhang
  • Chuan-Yih Yu
  • Ehwang Song
  • Yehia Mechref
  • Haixu Tang
  • Xiaowen Liu

Related Research Unit(s)

Detail(s)

Original languageEnglish
Pages (from-to)5099-5108
Journal / PublicationJournal of Proteome Research
Volume14
Issue number12
Publication statusPublished - 4 Dec 2015

Abstract

Glycosylation is one of the most common post-translational modifications in proteins, existing in ∼50% of mammalian proteins. Several research groups have demonstrated that mass spectrometry is an efficient technique for glycopeptide identification; however, this problem is still challenging because of the enormous diversity of glycan structures and the microheterogeneity of glycans. In addition, a glycopeptide may contain multiple glycosylation sites, making the problem complex. Current software tools often fail to identify glycopeptides with multiple glycosylation sites, and hence we present GlycoMID, a graph-based spectral alignment algorithm that can identify glycopeptides with multiple hydroxylysine O-glycosylation sites by tandem mass spectra. GlycoMID was tested on mass spectrometry data sets of the bovine collagen α-(II) chain protein, and experimental results showed that it identified more glycopeptide-spectrum matches than other existing tools, including many glycopeptides with two glycosylation sites.

Research Area(s)

  • Alignment algorithm, collagen α-(II) chain, glycopeptide identification, MS/MS spectra, O-linked glycosylation

Citation Format(s)

Identification of Glycopeptides with Multiple Hydroxylysine O-Glycosylation Sites by Tandem Mass Spectrometry. / Zhang, Yanlin; Yu, Chuan-Yih; Song, Ehwang; Li, Shuai Cheng; Mechref, Yehia; Tang, Haixu; Liu, Xiaowen.

In: Journal of Proteome Research, Vol. 14, No. 12, 04.12.2015, p. 5099-5108.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal