Identification of feline calicivirus in cats with enteritis

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

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Author(s)

  • Barbara Di Martino
  • Gianvito Lanave
  • Federica Di Profio
  • Irene Melegari
  • Fulvio Marsilio
  • Michele Camero
  • Cristiana Catella
  • Paolo Capozza
  • Krisztián Bányai
  • Canio Buonavoglia
  • Vito Martella

Detail(s)

Original languageEnglish
Journal / PublicationTransboundary and Emerging Diseases
Online published2 May 2020
Publication statusOnline published - 2 May 2020

Abstract

Feline calicivirus (FCV) is a major pathogen of cats associated with either respiratory disease or systemic disease, but its possible role as an enteric pathogen is neglected. Using RT-PCR, the RNA of FCV was identified in 25.9% (62/239) of stools of cats with enteritis and in 0/58 (0%) of cats without diarrhoea or other clinical signs. Isolates of enteric origin were obtained and a large 3.2-kb portion of the genome was sequenced, encompassing the 3′ end of the RNA polymerase, the capsid protein precursor and the minor capsid protein. Also, the complete genome sequence of one such strain, the 160/2015/ITA, was determined. Upon sequence analysis, the enteric viruses were found to be genetically heterogeneous and to differ from each other and from isolates of respiratory origin. The enteric isolates were found to be more resistant to low pH conditions, to trypsin and to bile treatment than respiratory isolates. Overall, these findings are consistent with the hypothesis that some FCVs may acquire enteric tropism and eventually act as enteric pathogens. Whether this enteric tropism is maintained stably and whether it may affect, to some extent, the ability of the virus to trigger the classical and/or hypervirulent forms of disease should be assessed. Also, FCV should be included in the diagnostic algorithms of enteric diseases of cats to gain further information about FCV strains displaying enteric pathotype.

Research Area(s)

  • cats, enteritis, feline calicivirus (FCV)

Citation Format(s)

Identification of feline calicivirus in cats with enteritis. / Di Martino, Barbara; Lanave, Gianvito; Di Profio, Federica; Melegari, Irene; Marsilio, Fulvio; Camero, Michele; Catella, Cristiana; Capozza, Paolo; Bányai, Krisztián; Barrs, Vanessa R.; Buonavoglia, Canio; Martella, Vito.

In: Transboundary and Emerging Diseases, 02.05.2020.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal