Identification of avian polyomavirus and its pathogenicity to SPF chickens

Tianshu Zhai; Jiajia Yan; Jia Wang; Dongni Kong; Lidan Hou; Yong Deng; Guoqian Gu; Tuanjie Wang; Xi Wang; Qinghong Xue; Chunsheng Yin; Jia Cheng; Guanlong Xu; Yaqing Mao

doi:10.3389/fmicb.2023.1320264

Identification of avian polyomavirus and its pathogenicity to SPF chickens

Tianshu Zhai, Jiajia Yan, Jia Wang, Dongni Kong, Lidan Hou, Yong Deng, Guoqian Gu, Tuanjie Wang, Xi Wang, Qinghong Xue, Chunsheng Yin, Jia Cheng^*, Guanlong Xu^*, Yaqing Mao^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

2 Citations (Scopus)

41 Downloads (CityUHK Scholars)

Abstract

The research aimed to study an Avian polyomavirus strain that was isolated in Shandong, China. To study the pathogenicity of APV in SPF chickens, and provide references for epidemiological research and disease prevention and control of APV. The genetic characterization of APV strain (termed APV-20) was analyzed and the pathogenicity of APV was investigated from two aspects: different age SPF chickens, and different infection doses. The results revealed that the APV-20 exhibits a nucleotide homology of 99% with the other three APV strains, and the evolution of APV In China was slow. In addition, the APV-20 infection in chickens caused depression, drowsiness, clustering, and fluffy feathers, but no deaths occurred in the infected chickens. The main manifestations of necropsy, and Hematoxylin and Eosin staining (HE) showed that one-day-old SPF chickens were the most susceptible, and there was a positive correlation between viral load and infection dose in the same tissue. This study showed that SPF chickens were susceptible to APV, and an experimental animal model was established. This study can provide a reference for the pathogenic mechanism of immune prevention and control of APV. © 2024 Zhai, Yan, Wang, Kong, Hou, Deng, Gu, Wang, Wang, Xue, Yin, Cheng, Xu and Mao.

Original language	English
Article number	1320264
Journal	Frontiers in Microbiology
Volume	14
DOIs	https://doi.org/10.3389/fmicb.2023.1320264
Publication status	Published - 2023

Research Keywords

avian polyomavirus
biological identification
SPF chicken
pathogenicity
VP4

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title = "Identification of avian polyomavirus and its pathogenicity to SPF chickens",

abstract = "The research aimed to study an Avian polyomavirus strain that was isolated in Shandong, China. To study the pathogenicity of APV in SPF chickens, and provide references for epidemiological research and disease prevention and control of APV. The genetic characterization of APV strain (termed APV-20) was analyzed and the pathogenicity of APV was investigated from two aspects: different age SPF chickens, and different infection doses. The results revealed that the APV-20 exhibits a nucleotide homology of 99% with the other three APV strains, and the evolution of APV In China was slow. In addition, the APV-20 infection in chickens caused depression, drowsiness, clustering, and fluffy feathers, but no deaths occurred in the infected chickens. The main manifestations of necropsy, and Hematoxylin and Eosin staining (HE) showed that one-day-old SPF chickens were the most susceptible, and there was a positive correlation between viral load and infection dose in the same tissue. This study showed that SPF chickens were susceptible to APV, and an experimental animal model was established. This study can provide a reference for the pathogenic mechanism of immune prevention and control of APV. {\textcopyright} 2024 Zhai, Yan, Wang, Kong, Hou, Deng, Gu, Wang, Wang, Xue, Yin, Cheng, Xu and Mao.",

keywords = "avian polyomavirus, biological identification, SPF chicken, pathogenicity, VP4",

author = "Tianshu Zhai and Jiajia Yan and Jia Wang and Dongni Kong and Lidan Hou and Yong Deng and Guoqian Gu and Tuanjie Wang and Xi Wang and Qinghong Xue and Chunsheng Yin and Jia Cheng and Guanlong Xu and Yaqing Mao",

year = "2023",

doi = "10.3389/fmicb.2023.1320264",

language = "English",

volume = "14",

journal = "Frontiers in Microbiology",

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TY - JOUR

T1 - Identification of avian polyomavirus and its pathogenicity to SPF chickens

AU - Zhai, Tianshu

AU - Yan, Jiajia

AU - Wang, Jia

AU - Kong, Dongni

AU - Hou, Lidan

AU - Deng, Yong

AU - Gu, Guoqian

AU - Wang, Tuanjie

AU - Wang, Xi

AU - Xue, Qinghong

AU - Yin, Chunsheng

AU - Cheng, Jia

AU - Xu, Guanlong

AU - Mao, Yaqing

PY - 2023

Y1 - 2023

N2 - The research aimed to study an Avian polyomavirus strain that was isolated in Shandong, China. To study the pathogenicity of APV in SPF chickens, and provide references for epidemiological research and disease prevention and control of APV. The genetic characterization of APV strain (termed APV-20) was analyzed and the pathogenicity of APV was investigated from two aspects: different age SPF chickens, and different infection doses. The results revealed that the APV-20 exhibits a nucleotide homology of 99% with the other three APV strains, and the evolution of APV In China was slow. In addition, the APV-20 infection in chickens caused depression, drowsiness, clustering, and fluffy feathers, but no deaths occurred in the infected chickens. The main manifestations of necropsy, and Hematoxylin and Eosin staining (HE) showed that one-day-old SPF chickens were the most susceptible, and there was a positive correlation between viral load and infection dose in the same tissue. This study showed that SPF chickens were susceptible to APV, and an experimental animal model was established. This study can provide a reference for the pathogenic mechanism of immune prevention and control of APV. © 2024 Zhai, Yan, Wang, Kong, Hou, Deng, Gu, Wang, Wang, Xue, Yin, Cheng, Xu and Mao.

AB - The research aimed to study an Avian polyomavirus strain that was isolated in Shandong, China. To study the pathogenicity of APV in SPF chickens, and provide references for epidemiological research and disease prevention and control of APV. The genetic characterization of APV strain (termed APV-20) was analyzed and the pathogenicity of APV was investigated from two aspects: different age SPF chickens, and different infection doses. The results revealed that the APV-20 exhibits a nucleotide homology of 99% with the other three APV strains, and the evolution of APV In China was slow. In addition, the APV-20 infection in chickens caused depression, drowsiness, clustering, and fluffy feathers, but no deaths occurred in the infected chickens. The main manifestations of necropsy, and Hematoxylin and Eosin staining (HE) showed that one-day-old SPF chickens were the most susceptible, and there was a positive correlation between viral load and infection dose in the same tissue. This study showed that SPF chickens were susceptible to APV, and an experimental animal model was established. This study can provide a reference for the pathogenic mechanism of immune prevention and control of APV. © 2024 Zhai, Yan, Wang, Kong, Hou, Deng, Gu, Wang, Wang, Xue, Yin, Cheng, Xu and Mao.

KW - avian polyomavirus

KW - biological identification

KW - SPF chicken

KW - pathogenicity

KW - VP4

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U2 - 10.3389/fmicb.2023.1320264

DO - 10.3389/fmicb.2023.1320264

M3 - RGC 21 - Publication in refereed journal

C2 - 38235429

SN - 1664-302X

VL - 14

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

M1 - 1320264

ER -

Identification of avian polyomavirus and its pathogenicity to SPF chickens

Abstract

Research Keywords

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