Identification of a Novel Betacoronavirus (Merbecovirus) in Amur Hedgehogs from China

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

9 Scopus Citations
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Author(s)

  • Susanna K. P. Lau
  • Hayes K. H. Luk
  • Antonio C. P. Wong
  • Rachel Y. Y. Fan
  • Carol S. F. Lam
  • Kenneth S. M. Li
  • Syed Shakeel Ahmed
  • Franklin W. N. Chow
  • Jian-Piao Cai
  • Xun Zhu
  • Jasper F. W. Chan
  • Kaiyuan Cao
  • Mengfeng Li
  • Patrick C. Y. Woo
  • Kwok-Yung Yuen

Detail(s)

Original languageEnglish
Journal / PublicationViruses
Volume11
Issue number11
Online published24 Oct 2019
Publication statusPublished - Nov 2019

Abstract

While dromedaries are the immediate animal source of Middle East Respiratory Syndrome (MERS) epidemic, viruses related to MERS coronavirus (MERS-CoV) have also been found in bats as well as hedgehogs. To elucidate the evolution of MERS-CoV-related viruses and their interspecies transmission pathway, samples were collected from different mammals in China. A novel coronavirus related to MERS-CoV, Erinaceus amurensis hedgehog coronavirus HKU31 (Ea-HedCoV HKU31), was identified from two Amur hedgehogs. Genome analysis supported that Ea-HedCoV HKU31 represents a novel species under Merbecovirus, being most closely related to Erinaceus CoV from European hedgehogs in Germany, with 79.6% genome sequence identity. Compared to other members of Merbecovirus, Ea-HedCoV HKU31 possessed unique non-structural proteins and putative cleavage sites at ORF1ab. Phylogenetic analysis showed that Ea-HedCoV HKU31 and BetaCoV Erinaceus/VMC/DEU/2012 were closely related to NeoCoV and BatCoV PREDICT from African bats in the spike region, suggesting that the latter bat viruses have arisen from recombination between CoVs from hedgehogs and bats. The predicted HKU31 receptor-binding domain (RBD) possessed only one out of 12 critical amino acid residues for binding to human dipeptidyl peptidase 4 (hDPP4), the MERS-CoV receptor. The structural modeling of the HKU31-RBD-hDPP4 binding interphase compared to that of MERS-CoV and Tylonycteris bat CoV HKU4 (Ty-BatCoV HKU4) suggested that HKU31-RBD is unlikely to bind to hDPP4. Our findings support that hedgehogs are an important reservoir of Merbecovirus, with evidence of recombination with viruses from bats. Further investigations in bats, hedgehogs and related animals are warranted to understand the evolution of MERS-CoV-related viruses.

Research Area(s)

  • China, coronavirus, hedgehog, Merbecovirus, novel species

Citation Format(s)

Identification of a Novel Betacoronavirus (Merbecovirus) in Amur Hedgehogs from China. / Lau, Susanna K. P.; Luk, Hayes K. H.; Wong, Antonio C. P.; Fan, Rachel Y. Y.; Lam, Carol S. F.; Li, Kenneth S. M.; Ahmed, Syed Shakeel; Chow, Franklin W. N.; Cai, Jian-Piao; Zhu, Xun; Chan, Jasper F. W.; Lau, Terrence C. K.; Cao, Kaiyuan; Li, Mengfeng; Woo, Patrick C. Y.; Yuen, Kwok-Yung.

In: Viruses, Vol. 11, No. 11, 11.2019.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal