Identification of a KPC Variant Conferring Resistance to Ceftazidime-Avibactam from ST11 Carbapenem-Resistant Klebsiella pneumoniae Strains

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Author(s)

  • Yuchen Wu
  • Congcong Liu
  • Yanyan Zhang
  • Edward Wai Chi Chan
  • Rong Zhang

Detail(s)

Original languageEnglish
Journal / PublicationMicrobiology Spectrum
Volume10
Issue number2
Publication statusPublished - Mar 2022

Link(s)

Abstract

A novel Klebsiella pneumoniae carbapenemase (KPC) variant, KPC-93, was identified in two Klebsiella pneumoniae clinical isolates from a patient from China treated with ceftazidime-avibactam. KPC-93 possessed a five-amino-acids insertion (Pro-Asn-Asn-Arg-Ala) between Ambler positions 267 and 268 in KPC-2. Cloning and expression of the blaKPC-93 gene in Escherichia coli, followed by determination of minimum inhibitory concentration (MIC) values and kinetic parameters, showed that KPC-93 exhibited increased resistance to ceftazidime-avibactam, but a drastic decrease in carbapenemase activity. Our data highlight that a KPC variant conferring resistance to ceftazidime-avibactam could be easily induced by ceftazidime-avibactam treatment and that actions are required to control dissemination of these determinants.

Research Area(s)

  • KPC, ceftazidime-avibactam, Klebsiella pneumoniae, SUSCEPTIBILITY, TOOL

Citation Format(s)

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