Identification and Characterization of Conjugative Plasmids That Encode Ciprofloxacin Resistance in Salmonella

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

6 Scopus Citations
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Author(s)

  • Kaichao Chen
  • Ning Dong
  • Shaohua Zhao
  • Lizhang Liu
  • Ruichao Li
  • Miaomiao Xie
  • Dachuan Lin
  • Edward Wai-Chi Chan
  • Jianghong Meng
  • Patrick F. McDermott

Detail(s)

Original languageEnglish
Journal / PublicationAntimicrobial Agents and Chemotherapy
Volume62
Issue number8
Online published14 May 2018
Publication statusPublished - Aug 2018
Externally publishedYes

Abstract

This study aimed to characterize novel conjugative plasmids that encode transferable ciprofloxacin resistance in Salmonella. In this study, 157 nonduplicated Salmonella isolates were recovered from food products, of which 55 were found to be resistant to ciprofloxacin. Interestingly, 37 of the 55 Cip(r) Salmonella isolates (67%) did not harbor any mutations in the quinolone resistance-determining regions (QRDR). Six Salmonella isolates were shown to carry two novel types of conjugative plasmids that could transfer the ciprofloxacin resistance phenotype to Escherichia coli J53 (azithromycin resistant [Azi(r)]). The first type of conjugative plasmid belonged to the similar to 110-kb IncFIB-type conjugative plasmids carrying qnrB-bearing and aac(6')-lb-cr-bearing mobile elements. Transfer of the plasmid between E. coli and Salmonella could confer a ciprofloxacin MIC of 1 to 2 mu g/ml. The second type of conjugative plasmid belonged to similar to 240-kb IncH1/IncF plasmids carrying a single PMQR gene, qnrS. Importantly, this type of conjugative ciprofloxacin resistance plasmid could be detected in clinical Salmonella isolates. The dissemination of these conjugative plasmids that confer ciprofloxacin resistance poses serious challenges to public health and Salmonella infection control.

Research Area(s)

  • PMQR genes, Salmonella, ciprofloxacin resistance, conjugative plasmid, MEDIATED QUINOLONE RESISTANCE, ESCHERICHIA-COLI, FLUOROQUINOLONE RESISTANCE, RETAIL MEATS, ENTERICA, TYPHIMURIUM, MECHANISMS, HUMANS, PREVALENCE, EMERGENCE

Citation Format(s)

Identification and Characterization of Conjugative Plasmids That Encode Ciprofloxacin Resistance in Salmonella. / Chen, Kaichao; Dong, Ning; Zhao, Shaohua; Liu, Lizhang; Li, Ruichao; Xie, Miaomiao; Lin, Dachuan; Chan, Edward Wai-Chi; Meng, Jianghong; McDermott, Patrick F.; Chen, Sheng.

In: Antimicrobial Agents and Chemotherapy, Vol. 62, No. 8, 08.2018.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal