Hypoxia-induced epigenetic transgenerational miRNAs dysregulation involved in reproductive impairment of ovary

Keng Po Lai; Cherry Chi Tim Leung; Delbert Almerick T. Boncan; Nathan Tam; Xiao Lin; Simon Yuan Wang; Ting Fung Chan; Rudolf Shiu Sun Wu; Richard Yuen Chong Kong

doi:10.1016/j.cbi.2022.110176

Hypoxia-induced epigenetic transgenerational miRNAs dysregulation involved in reproductive impairment of ovary

Keng Po Lai^*, Cherry Chi Tim Leung, Delbert Almerick T. Boncan, Nathan Tam, Xiao Lin, Simon Yuan Wang, Ting Fung Chan, Rudolf Shiu Sun Wu, Richard Yuen Chong Kong^*

^*Corresponding author for this work

Department of Chemistry

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

8 Citations (Scopus)

Abstract

Hypoxia is a potent endocrine disruptor that is posing serious problems to the fish reproductive systems. Our previous studies reported that hypoxia could cause a transgenerational impairment of ovarian development and interfere hatching success in F2 offspring of marine medaka fish (Oryzias melastigma) through epigenetic regulation. As part of the epigenetic regulation, we investigated the involvement of microRNAs (miRNAs) in hypoxia-induced transgenerational reproductive impairments. In the present study, we used comparative small RNA sequencing to reveal that hypoxia caused miRNA dysregulation in ovaries of F0 hypoxia group and F2 transgenerational group. We found 4 common dysregulated miRNA in the F0 and F2 generations. Furthermore, integrated miRNA-mRNA analysis, followed by gene ontology enrichment analysis on the hypoxia-dysregulated miRNA-target genes further highlighted the importance of these dysregulated miRNAs in biological processes related to reproduction. More importantly, we identified 3 miRNA-mRNA pairs (novel miRNA-525-DIAPH2, novel miRNA-525-MYOCD, and novel miRNA-525-RAI14) that might play epigenetic roles in hypoxia-induced reproductive impairment. For the first time, our findings suggested the involvement of miRNA in hypoxia-induced reproductive impairments may be inherited via a transgenerational manner.

Original language	English
Article number	110176
Journal	Chemico-Biological Interactions
Volume	367
Online published	10 Sept 2022
DOIs	https://doi.org/10.1016/j.cbi.2022.110176
Publication status	Published - 1 Nov 2022

Funding

This work was financially supported by a General Research Fund (CityU 11104520) to RYCK and a CRF grant (SKLMP/CRF/0004) of the State Key Laboratory of Marine Pollution of the City University of Hong Kong to RSSW.

Research Keywords

Hypoxia
miRNA
Ovary
Transcriptome
Transgenerational

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RGC-funded

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10.1016/j.cbi.2022.110176

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title = "Hypoxia-induced epigenetic transgenerational miRNAs dysregulation involved in reproductive impairment of ovary",

abstract = "Hypoxia is a potent endocrine disruptor that is posing serious problems to the fish reproductive systems. Our previous studies reported that hypoxia could cause a transgenerational impairment of ovarian development and interfere hatching success in F2 offspring of marine medaka fish (Oryzias melastigma) through epigenetic regulation. As part of the epigenetic regulation, we investigated the involvement of microRNAs (miRNAs) in hypoxia-induced transgenerational reproductive impairments. In the present study, we used comparative small RNA sequencing to reveal that hypoxia caused miRNA dysregulation in ovaries of F0 hypoxia group and F2 transgenerational group. We found 4 common dysregulated miRNA in the F0 and F2 generations. Furthermore, integrated miRNA-mRNA analysis, followed by gene ontology enrichment analysis on the hypoxia-dysregulated miRNA-target genes further highlighted the importance of these dysregulated miRNAs in biological processes related to reproduction. More importantly, we identified 3 miRNA-mRNA pairs (novel miRNA-525-DIAPH2, novel miRNA-525-MYOCD, and novel miRNA-525-RAI14) that might play epigenetic roles in hypoxia-induced reproductive impairment. For the first time, our findings suggested the involvement of miRNA in hypoxia-induced reproductive impairments may be inherited via a transgenerational manner.",

keywords = "Hypoxia, miRNA, Ovary, Transcriptome, Transgenerational",

author = "Lai, {Keng Po} and {Tim Leung}, {Cherry Chi} and Boncan, {Delbert Almerick T.} and Nathan Tam and Xiao Lin and Wang, {Simon Yuan} and Chan, {Ting Fung} and {Sun Wu}, {Rudolf Shiu} and {Chong Kong}, {Richard Yuen}",

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doi = "10.1016/j.cbi.2022.110176",

language = "English",

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journal = "Chemico-Biological Interactions",

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publisher = "Elsevier Ireland Ltd",

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TY - JOUR

T1 - Hypoxia-induced epigenetic transgenerational miRNAs dysregulation involved in reproductive impairment of ovary

AU - Lai, Keng Po

AU - Tim Leung, Cherry Chi

AU - Boncan, Delbert Almerick T.

AU - Tam, Nathan

AU - Lin, Xiao

AU - Wang, Simon Yuan

AU - Chan, Ting Fung

AU - Sun Wu, Rudolf Shiu

AU - Chong Kong, Richard Yuen

PY - 2022/11/1

Y1 - 2022/11/1

N2 - Hypoxia is a potent endocrine disruptor that is posing serious problems to the fish reproductive systems. Our previous studies reported that hypoxia could cause a transgenerational impairment of ovarian development and interfere hatching success in F2 offspring of marine medaka fish (Oryzias melastigma) through epigenetic regulation. As part of the epigenetic regulation, we investigated the involvement of microRNAs (miRNAs) in hypoxia-induced transgenerational reproductive impairments. In the present study, we used comparative small RNA sequencing to reveal that hypoxia caused miRNA dysregulation in ovaries of F0 hypoxia group and F2 transgenerational group. We found 4 common dysregulated miRNA in the F0 and F2 generations. Furthermore, integrated miRNA-mRNA analysis, followed by gene ontology enrichment analysis on the hypoxia-dysregulated miRNA-target genes further highlighted the importance of these dysregulated miRNAs in biological processes related to reproduction. More importantly, we identified 3 miRNA-mRNA pairs (novel miRNA-525-DIAPH2, novel miRNA-525-MYOCD, and novel miRNA-525-RAI14) that might play epigenetic roles in hypoxia-induced reproductive impairment. For the first time, our findings suggested the involvement of miRNA in hypoxia-induced reproductive impairments may be inherited via a transgenerational manner.

AB - Hypoxia is a potent endocrine disruptor that is posing serious problems to the fish reproductive systems. Our previous studies reported that hypoxia could cause a transgenerational impairment of ovarian development and interfere hatching success in F2 offspring of marine medaka fish (Oryzias melastigma) through epigenetic regulation. As part of the epigenetic regulation, we investigated the involvement of microRNAs (miRNAs) in hypoxia-induced transgenerational reproductive impairments. In the present study, we used comparative small RNA sequencing to reveal that hypoxia caused miRNA dysregulation in ovaries of F0 hypoxia group and F2 transgenerational group. We found 4 common dysregulated miRNA in the F0 and F2 generations. Furthermore, integrated miRNA-mRNA analysis, followed by gene ontology enrichment analysis on the hypoxia-dysregulated miRNA-target genes further highlighted the importance of these dysregulated miRNAs in biological processes related to reproduction. More importantly, we identified 3 miRNA-mRNA pairs (novel miRNA-525-DIAPH2, novel miRNA-525-MYOCD, and novel miRNA-525-RAI14) that might play epigenetic roles in hypoxia-induced reproductive impairment. For the first time, our findings suggested the involvement of miRNA in hypoxia-induced reproductive impairments may be inherited via a transgenerational manner.

KW - Hypoxia

KW - miRNA

KW - Ovary

KW - Transcriptome

KW - Transgenerational

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ER -

Hypoxia-induced epigenetic transgenerational miRNAs dysregulation involved in reproductive impairment of ovary

Abstract

Funding

Research Keywords

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GRF: Interactive Effects of 17α-ethinylestradiol (EE2) and Hypoxia on Impairment of Testicular Functions in Male Marine Medaka Fish

Cite this

Hypoxia-induced epigenetic transgenerational miRNAs dysregulation involved in reproductive impairment of ovary

Abstract

Funding

Research Keywords

RGC Funding Information

Access Output

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Permanent Link

Other Files and Links

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GRF: Interactive Effects of 17α-ethinylestradiol (EE2) and Hypoxia on Impairment of Testicular Functions in Male Marine Medaka Fish

Cite this