Hydrogel Stamping for Rapid, Multiplexed, Point-of-Care Immunostaining of Cells and Tissues

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Huiyan Li
  • Jae-Hyeok Choi
  • Yoshiko Iwamoto
  • Juhyun Oh
  • Jouha Min
  • Suk Kyung Beak
  • Dahyeon Yoo
  • Cesar M. Castro
  • Dongyoung Lee
  • Hyungsoon Im

Related Research Unit(s)

Detail(s)

Original languageEnglish
Pages (from-to)27613-27622
Number of pages10
Journal / PublicationACS Applied Materials and Interfaces
Volume14
Issue number24
Online published7 Jun 2022
Publication statusPublished - 22 Jun 2022

Abstract

In the era of precision oncology, multicolor fluorescence imaging has become a core technology for multiplexed molecular analysis of cellular and tissue specimens. However, conventional solution-based staining is labor-intensive and time-consuming and requires considerable expertise to yield optimal results, which creates difficulties for employing this technology in resource-limited settings. Here, we report a new immunostaining method based on hydrogel stamping, which is simple, fast, easy to use, and reproducible. We showed that a hydrophilic hydrogel stamp could effectively transfer fluorescent antibodies to targets and withdraw an excess solution when the reaction is completed, obviating the need for extra washing. This unique property allows for quality immunostaining in 5 min for cells using one-eighth of antibody consumption compared to the conventional solution-based method. Furthermore, we implemented fluorescence quenching and immunocycling with hydrogel staining for multiplexed analysis of 9 protein markers at a single cell level. Finally, we applied the immunocycling method to human breast cancer tissue samples and showed quality immunostaining over a large area (∼2 cm2) in 30 min for molecular subtyping of breast cancer. The hydrogel immunostaining could open new opportunities for rapid, automated, and multiplexed profiling in compact point-of-care systems for molecular cancer diagnosis.

Research Area(s)

  • hydrogel, immunostaining, cancer, pathology, diagnostics, molecualr profiling

Citation Format(s)

Hydrogel Stamping for Rapid, Multiplexed, Point-of-Care Immunostaining of Cells and Tissues. / Chin, Lip Ket; Li, Huiyan; Choi, Jae-Hyeok; Iwamoto, Yoshiko; Oh, Juhyun; Min, Jouha; Beak, Suk Kyung; Yoo, Dahyeon; Castro, Cesar M.; Lee, Dongyoung; Im, Hyungsoon.

In: ACS Applied Materials and Interfaces, Vol. 14, No. 24, 22.06.2022, p. 27613-27622.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review