Hydrodynamic Focusing for a Micromachined Flow Cytometer

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Chen-I. Hung
  • Bin-Jo Ke
  • Guan-Ruey Huang
  • Bao-Herng Hwei
  • Hui-Fang Lai

Detail(s)

Original languageEnglish
Pages (from-to)672-679
Journal / PublicationJournal of Fluids Engineering, Transactions of the ASME
Volume123
Issue number3
Online published18 Apr 2001
Publication statusPublished - Sep 2001
Externally publishedYes

Abstract

This paper describes hydrodynamic focusing inside a micromachined flow cytometer. Flow cytometry is a process whereby cells are analyzed and sorted based on hydrodynamic focusing phenomenon and specific cellular characteristics. In this study, the hydrodynamic focusing phenomenon is first modeled by employing potential flow theory. Then the flow field inside the flow cytometer is numerically simulated. The effect of the device geometry and relative sheath and sample flow rate on the focusing of the center flow is explored systematically. At last, a micromachine-based flow chamber is designed and fabricated on plastic substrates as a micro flow cytometer. Hydrodynamic focusing is verified with the use of microscopic visualization of water sheath flows and dyecontaining sample flow. Experimental data indicate that the size of focused sample stream can be reduced to about 3 μm, which is applicable to cell sorting and counting. © 2001 by ASME.

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Citation Format(s)

Hydrodynamic Focusing for a Micromachined Flow Cytometer. / Lee, Gwo-Bin; Hung, Chen-I.; Ke, Bin-Jo; Huang, Guan-Ruey; Hwei, Bao-Herng; Lai, Hui-Fang.

In: Journal of Fluids Engineering, Transactions of the ASME, Vol. 123, No. 3, 09.2001, p. 672-679.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review