TY - JOUR
T1 - Hydrodynamic Focusing for a Micromachined Flow Cytometer
AU - Lee, Gwo-Bin
AU - Hung, Chen-I.
AU - Ke, Bin-Jo
AU - Huang, Guan-Ruey
AU - Hwei, Bao-Herng
AU - Lai, Hui-Fang
N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].
PY - 2001/9
Y1 - 2001/9
N2 - This paper describes hydrodynamic focusing inside a micromachined flow cytometer. Flow cytometry is a process whereby cells are analyzed and sorted based on hydrodynamic focusing phenomenon and specific cellular characteristics. In this study, the hydrodynamic focusing phenomenon is first modeled by employing potential flow theory. Then the flow field inside the flow cytometer is numerically simulated. The effect of the device geometry and relative sheath and sample flow rate on the focusing of the center flow is explored systematically. At last, a micromachine-based flow chamber is designed and fabricated on plastic substrates as a micro flow cytometer. Hydrodynamic focusing is verified with the use of microscopic visualization of water sheath flows and dyecontaining sample flow. Experimental data indicate that the size of focused sample stream can be reduced to about 3 μm, which is applicable to cell sorting and counting. © 2001 by ASME.
AB - This paper describes hydrodynamic focusing inside a micromachined flow cytometer. Flow cytometry is a process whereby cells are analyzed and sorted based on hydrodynamic focusing phenomenon and specific cellular characteristics. In this study, the hydrodynamic focusing phenomenon is first modeled by employing potential flow theory. Then the flow field inside the flow cytometer is numerically simulated. The effect of the device geometry and relative sheath and sample flow rate on the focusing of the center flow is explored systematically. At last, a micromachine-based flow chamber is designed and fabricated on plastic substrates as a micro flow cytometer. Hydrodynamic focusing is verified with the use of microscopic visualization of water sheath flows and dyecontaining sample flow. Experimental data indicate that the size of focused sample stream can be reduced to about 3 μm, which is applicable to cell sorting and counting. © 2001 by ASME.
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U2 - 10.1115/1.1385514
DO - 10.1115/1.1385514
M3 - RGC 21 - Publication in refereed journal
SN - 0098-2202
VL - 123
SP - 672
EP - 679
JO - Journal of Fluids Engineering, Transactions of the ASME
JF - Journal of Fluids Engineering, Transactions of the ASME
IS - 3
ER -