Hybrid capillary-inserted microfluidic device for sheathless particle focusing and separation in viscoelastic flow

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Jeonghun Nam
  • Justin Kok Soon Tan
  • Bumseok Namgung
  • Hwa Liang Leo
  • Chwee Teck Lim
  • Sangho Kim

Detail(s)

Original languageEnglish
Article number064117
Journal / PublicationBiomicrofluidics
Volume9
Issue number6
Publication statusPublished - Nov 2015
Externally publishedYes

Abstract

A novel microfluidic device which consists of two stages for particle focusing and separation using a viscoelastic fluid has been developed. A circular capillary tube was used for three-dimensional particle pre-alignment before the separation process, which was inserted in a polydimethylsiloxane microchannel. Particles with diameters of 5 and 10 μm were focused at the centerline in the capillary tube, and the location of particles was initialized at the first bifurcation. Then, 5 and 10 μm particles were successfully separated in the expansion region based on size-dependent lateral migration, with ~99% separation efficiency. The proposed device was further applied to separation of MCF-7 cells from leukocytes. Based on the cell size distribution, an approximate size cutoff for separation was determined to be 16 μm. At 200 μl/min, 94% of MCF-7 cells were separated with the purity of ~97%. According to the trypan blue exclusion assay, high viability (~90%) could be achieved for the separated MCF-7 cells. The use of a commercially available capillary tube enables the device to be highly versatile in dealing with particles in a wide size range by using capillary tubes with different inner diameters.

Citation Format(s)

Hybrid capillary-inserted microfluidic device for sheathless particle focusing and separation in viscoelastic flow. / Nam, Jeonghun; Tan, Justin Kok Soon; Khoo, Bee Luan et al.

In: Biomicrofluidics, Vol. 9, No. 6, 064117, 11.2015.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review