Humic substances as electron acceptors for anaerobic oxidation of methane driven by ANME-2d

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalNot applicablepeer-review

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Author(s)

  • Ya-Nan Bai
  • Xiu-Ning Wang
  • Jun Wu
  • Yong-Ze Lu
  • Liang Fu
  • Fang Zhang
  • Raymond J. Zeng

Detail(s)

Original languageEnglish
Article number114935
Journal / PublicationWater Research
Volume164
Early online date30 Jul 2019
Publication statusPublished - 1 Nov 2019

Abstract

Humic substances (humics) are ubiquitous in terrestrial and aquatic environments where they can serve as electron acceptors for anaerobic oxidation of organic compounds. Methane is a powerful greenhouse gas, as well as the least reactive organic molecule. Anaerobic oxidation of methane (AOM) coupled to microbial reduction of various electron acceptors plays a crucial role in mitigating methane emissions. Here, we reported that humics could serve as terminal electron acceptors for AOM using enriched nitrate-reducing AOM microorganisms. AOM coupled to the reduction of humics was demonstrated based on the production of 13C-labelled carbon dioxide, and AOM activity was evaluated with different methane partial pressures and electron acceptor concentrations. After three-cycle reduction, both AOM activity and copy numbers of the archaea 16S rRNA and mcrA genes were the highest when anthraquinone-2,6-disulfonic acid and anthraquinone-2-sulfonic acid were electron acceptors. The high-throughput sequencing results suggested that ANME-2d were the dominant methane oxidation archaea after humics reduction, although the partner bacteria NC10 trended downward, other reported humics reduction bacteria (Geobactor and Anammox) appeared. The potential electron transfer models from ANME-2d to humics were proposed. These results enable a better understanding of available electron acceptors for AOM in natural environments and broaden our insight into the significant role of ANME-2d.

Research Area(s)

  • Anaerobic oxidation of methane (AOM), ANME-2d, Electron acceptors, Humics, Methane

Citation Format(s)

Humic substances as electron acceptors for anaerobic oxidation of methane driven by ANME-2d. / Bai, Ya-Nan; Wang, Xiu-Ning; Wu, Jun; Lu, Yong-Ze; Fu, Liang; Zhang, Fang; Lau, Tai-Chu; Zeng, Raymond J.

In: Water Research, Vol. 164, 114935, 01.11.2019.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalNot applicablepeer-review