Human Mesenchymal Stem-Cell Behaviour On Direct Laser Micropatterned Electrospun Scaffolds with Hierarchical Structures

Huaqiong Li; Yee Shan Wong; Feng Wen; Kee Woei Ng; Gary Ka Lai Ng; Subbu S. Venkatraman; Freddy Yin Chiang Boey; Lay Poh Tan

doi:10.1002/mabi.201200318

Human Mesenchymal Stem-Cell Behaviour On Direct Laser Micropatterned Electrospun Scaffolds with Hierarchical Structures

Huaqiong Li, Yee Shan Wong, Feng Wen, Kee Woei Ng, Gary Ka Lai Ng, Subbu S. Venkatraman, Freddy Yin Chiang Boey, Lay Poh Tan^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

52 Citations (Scopus)

Abstract

Direct laser machining and electrospinning are utilized to obtain a bi-layered hybrid scaffold with hierarchical topographical features to mimic extracellular matrix-like microenvironment of cells. Adult bone marrow derived human mesenchymal stem cells (hMSCs) are cultured in vitro in these hybrid scaffolds, and cell orientation, proliferation, viability, and differentiation are evaluated. The results show that this novel hybrid scaffold not only supports cell growth like traditional scaffolds, but also elicits positive responses from the cells, like lineage commitment and alignment, which are essential features of future scaffolds. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Original language	English
Pages (from-to)	299-310
Journal	Macromolecular Bioscience
Volume	13
Issue number	3
Online published	11 Dec 2012
DOIs	https://doi.org/10.1002/mabi.201200318
Publication status	Published - Mar 2013
Externally published	Yes

Research Keywords

Electrospinning
High cell viability
Hybrid structures
Laser machining
Stem-cell differentiation

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10.1002/mabi.201200318

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title = "Human Mesenchymal Stem-Cell Behaviour On Direct Laser Micropatterned Electrospun Scaffolds with Hierarchical Structures",

abstract = "Direct laser machining and electrospinning are utilized to obtain a bi-layered hybrid scaffold with hierarchical topographical features to mimic extracellular matrix-like microenvironment of cells. Adult bone marrow derived human mesenchymal stem cells (hMSCs) are cultured in vitro in these hybrid scaffolds, and cell orientation, proliferation, viability, and differentiation are evaluated. The results show that this novel hybrid scaffold not only supports cell growth like traditional scaffolds, but also elicits positive responses from the cells, like lineage commitment and alignment, which are essential features of future scaffolds. {\textcopyright} 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.",

keywords = "Electrospinning, High cell viability, Hybrid structures, Laser machining, Stem-cell differentiation",

author = "Huaqiong Li and Wong, {Yee Shan} and Feng Wen and Ng, {Kee Woei} and Ng, {Gary Ka Lai} and Venkatraman, {Subbu S.} and Boey, {Freddy Yin Chiang} and Tan, {Lay Poh}",

year = "2013",

month = mar,

doi = "10.1002/mabi.201200318",

language = "English",

volume = "13",

pages = "299--310",

journal = "Macromolecular Bioscience",

issn = "1616-5187",

publisher = "Wiley-VCH Verlag GmbH",

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TY - JOUR

T1 - Human Mesenchymal Stem-Cell Behaviour On Direct Laser Micropatterned Electrospun Scaffolds with Hierarchical Structures

AU - Li, Huaqiong

AU - Wong, Yee Shan

AU - Wen, Feng

AU - Ng, Kee Woei

AU - Ng, Gary Ka Lai

AU - Venkatraman, Subbu S.

AU - Boey, Freddy Yin Chiang

AU - Tan, Lay Poh

PY - 2013/3

Y1 - 2013/3

N2 - Direct laser machining and electrospinning are utilized to obtain a bi-layered hybrid scaffold with hierarchical topographical features to mimic extracellular matrix-like microenvironment of cells. Adult bone marrow derived human mesenchymal stem cells (hMSCs) are cultured in vitro in these hybrid scaffolds, and cell orientation, proliferation, viability, and differentiation are evaluated. The results show that this novel hybrid scaffold not only supports cell growth like traditional scaffolds, but also elicits positive responses from the cells, like lineage commitment and alignment, which are essential features of future scaffolds. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

AB - Direct laser machining and electrospinning are utilized to obtain a bi-layered hybrid scaffold with hierarchical topographical features to mimic extracellular matrix-like microenvironment of cells. Adult bone marrow derived human mesenchymal stem cells (hMSCs) are cultured in vitro in these hybrid scaffolds, and cell orientation, proliferation, viability, and differentiation are evaluated. The results show that this novel hybrid scaffold not only supports cell growth like traditional scaffolds, but also elicits positive responses from the cells, like lineage commitment and alignment, which are essential features of future scaffolds. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

KW - Electrospinning

KW - High cell viability

KW - Hybrid structures

KW - Laser machining

KW - Stem-cell differentiation

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UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-84875519507&origin=recordpage

U2 - 10.1002/mabi.201200318

DO - 10.1002/mabi.201200318

M3 - RGC 21 - Publication in refereed journal

C2 - 23233197

SN - 1616-5187

VL - 13

SP - 299

EP - 310

JO - Macromolecular Bioscience

JF - Macromolecular Bioscience

IS - 3

ER -

Human Mesenchymal Stem-Cell Behaviour On Direct Laser Micropatterned Electrospun Scaffolds with Hierarchical Structures

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