TY - JOUR
T1 - Human epithelial cancers secrete immunoglobulin G with unidentified specificity to promote growth and survival of tumor cells
AU - Qiu, Xiaoyan
AU - Zhu, Xiaohui
AU - Zhang, Liang
AU - Mao, Yuntao
AU - Zhang, Jian
AU - Hao, Peng
AU - Li, Guohui
AU - Lv, Peng
AU - Li, Zhixin
AU - Sun, Xin
AU - Wu, Lemeng
AU - Zheng, Jie
AU - Deng, Yuqing
AU - Hou, Chunmei
AU - Tang, Peixian
AU - Zhang, Shuren
AU - Zhang, Youhui
PY - 2003/10
Y1 - 2003/10
N2 - Immunoglobulins (Igs) are found thus far only to be produced by differentiated B lymphocytes. By immunohistochemistry analysis, in situ hybridization, and laser capture microdissection-assisted single-cell PCR, we demonstrate that human cancers of epithelial origin, including carcinomas of breast, colon, liver, lung, established epithelial cancer lines, as well as some normal lung tissues, also produce IgG in both cytoplasmic and secreted forms. Furthermore, blockade of tumor-derived IgG by either antisense DNA or antihuman IgG antibody increased programmed cell death and inhibited growth of cancer cells in vitro. More importantly, administration of antihuman IgG antibody also suppressed the growth of an IgG-secreting carcinoma line in immunodeficient nude mice. Our results support a role of tumor-derived IgG as growth factor for epithelial cancers. Prevalent expression of IgG in human carcinomas and its growth-promoting functions may have important implications in growth regulation and targeted therapy of human cancers.
AB - Immunoglobulins (Igs) are found thus far only to be produced by differentiated B lymphocytes. By immunohistochemistry analysis, in situ hybridization, and laser capture microdissection-assisted single-cell PCR, we demonstrate that human cancers of epithelial origin, including carcinomas of breast, colon, liver, lung, established epithelial cancer lines, as well as some normal lung tissues, also produce IgG in both cytoplasmic and secreted forms. Furthermore, blockade of tumor-derived IgG by either antisense DNA or antihuman IgG antibody increased programmed cell death and inhibited growth of cancer cells in vitro. More importantly, administration of antihuman IgG antibody also suppressed the growth of an IgG-secreting carcinoma line in immunodeficient nude mice. Our results support a role of tumor-derived IgG as growth factor for epithelial cancers. Prevalent expression of IgG in human carcinomas and its growth-promoting functions may have important implications in growth regulation and targeted therapy of human cancers.
UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-0141988676&origin=recordpage
UR - http://www.scopus.com/inward/record.url?scp=0141988676&partnerID=8YFLogxK
M3 - RGC 21 - Publication in refereed journal
C2 - 14559841
SN - 0008-5472
VL - 63
SP - 6488
EP - 6495
JO - Cancer Research
JF - Cancer Research
IS - 19
ER -