TY - JOUR
T1 - HrpS is a global regulator on Type III Secretion System (T3SS) and non-T3SS genes in pseudomonas savastanoi pv. phaseolicola
AU - Wang, Jingru
AU - Shao, Xiaolong
AU - Zhang, Yingchao
AU - Zhu, Yanan
AU - Yang, Pan
AU - Yuan, Jian
AU - Wang, Tingting
AU - Yin, Chunyan
AU - Wang, Wei
AU - Chen, Sheng
AU - Liang, Haihua
AU - Deng, Xin
PY - 2018/12
Y1 - 2018/12
N2 - The type III secretion system (T3SS) is the main machinery for Pseudomonas savastanoi and other gram-negative bacteria to invade plant cells. HrpR and HrpS form a hetero-hexamer, which activates the expression of HrpL, which induces all T3SS genes by binding to a ‘hrp box’ in promoters. However, the individual molecular mechanism of HrpR or HrpS has not been fully understood. Through chromatin immunoprecipitation coupled to high-throughput DNA sequencing, we found that HrpR, HrpS, and HrpL had four, 47, and 31 targets on the genome, respectively. HrpS directly bound to the promoter regions of a group of T3SS genes and non-T3SS genes. HrpS independently regulated these genes in a hrpL deletion strain. Additionally, a HrpS-binding motif (GTGCCAAA) was identified, which was verified by electrophoretic mobility shift assay and lux-reporter assay. HrpS also regulated motility and biofilm formation in P. savastanoi. The present study strongly suggests that HrpS alone can work as a global regulator on both T3SS and non-T3SS genes in P. savastanoi.
AB - The type III secretion system (T3SS) is the main machinery for Pseudomonas savastanoi and other gram-negative bacteria to invade plant cells. HrpR and HrpS form a hetero-hexamer, which activates the expression of HrpL, which induces all T3SS genes by binding to a ‘hrp box’ in promoters. However, the individual molecular mechanism of HrpR or HrpS has not been fully understood. Through chromatin immunoprecipitation coupled to high-throughput DNA sequencing, we found that HrpR, HrpS, and HrpL had four, 47, and 31 targets on the genome, respectively. HrpS directly bound to the promoter regions of a group of T3SS genes and non-T3SS genes. HrpS independently regulated these genes in a hrpL deletion strain. Additionally, a HrpS-binding motif (GTGCCAAA) was identified, which was verified by electrophoretic mobility shift assay and lux-reporter assay. HrpS also regulated motility and biofilm formation in P. savastanoi. The present study strongly suggests that HrpS alone can work as a global regulator on both T3SS and non-T3SS genes in P. savastanoi.
UR - http://www.scopus.com/inward/record.url?scp=85056656616&partnerID=8YFLogxK
UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85056656616&origin=recordpage
U2 - 10.1094/MPMI-02-18-0035-R
DO - 10.1094/MPMI-02-18-0035-R
M3 - RGC 21 - Publication in refereed journal
C2 - 29717915
SN - 0894-0282
VL - 31
SP - 1232
EP - 1243
JO - Molecular Plant-Microbe Interactions
JF - Molecular Plant-Microbe Interactions
IS - 12
ER -