Hollow superparamagnetic iron oxide nanoshells as a hydrophobic anticancer drug carrier : Intracelluar pH-dependent drug release and enhanced cytotoxicity

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Xiao-Ming Zhu
  • Jing Yuan
  • Ken Cham-Fai Leung
  • Siu-Fung Lee
  • Kathy W. Y. Sham
  • Christopher H. K. Cheng
  • Gao-Jun Teng
  • Anil T. Ahuja
  • Yi-Xiang J. Wang

Detail(s)

Original languageEnglish
Pages (from-to)5744-5754
Journal / PublicationNanoscale
Volume4
Issue number18
Publication statusPublished - 21 Sep 2012

Abstract

With curcumin and doxorubicin (DOX) base as model drugs, intracellular delivery of hydrophobic anticancer drugs by hollow structured superparamagnetic iron oxide (SPIO) nanoshells (hydrodynamic diameter: 191.9 ± 2.6 nm) was studied in glioblastoma U-87 MG cells. SPIO nanoshell-based encapsulation provided a stable aqueous dispersion of the curcumin. After the SPIO nanoshells were internalized by U-87 MG cells, they localized at the acidic compartments of endosomes and lysosomes. In endosome/lysosome-mimicking buffers with a pH of 4.5-5.5, pH-dependent drug release was observed from curcumin or DOX loaded SPIO nanoshells (curcumin/SPIO or DOX/SPIO). Compared with the free drug, the intracellular curcumin content delivered via curcumin/SPIO was 30 fold higher. Increased intracellular drug content for DOX base delivered via DOX/SPIO was also confirmed, along with a fast intracellular DOX release that was attributed to its protonation in the acidic environment. DOX/SPIO enhanced caspase-3 activity by twofold compared with free DOX base. The concentration that induced 50% cytotoxic effect (CC50) was 0.05 ± 0.03 μg ml -1 for DOX/SPIO, while it was 0.13 ± 0.02 μg ml -1 for free DOX base. These results suggested SPIO nanoshells might be a promising intracellular carrier for hydrophobic anticancer drugs. © The Royal Society of Chemistry 2012.

Citation Format(s)

Hollow superparamagnetic iron oxide nanoshells as a hydrophobic anticancer drug carrier : Intracelluar pH-dependent drug release and enhanced cytotoxicity. / Zhu, Xiao-Ming; Yuan, Jing; Leung, Ken Cham-Fai; Lee, Siu-Fung; Sham, Kathy W. Y.; Cheng, Christopher H. K.; Au, Doris W. T.; Teng, Gao-Jun; Ahuja, Anil T.; Wang, Yi-Xiang J.

In: Nanoscale, Vol. 4, No. 18, 21.09.2012, p. 5744-5754.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review