Histone H3 lysine 4 monomethylation modulates long-range chromatin interactions at enhancers

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

31 Scopus Citations
View graph of relations

Author(s)

  • Shi-An A. Chen
  • Andrea Local
  • Tristin Liu
  • Yunjiang Qiu
  • Kristel M. Dorighi
  • Sebastian Preissl
  • Chloe M. Rivera
  • Chaochen Wang
  • Zhen Ye
  • Kai Ge
  • Ming Hu
  • Joanna Wysocka
  • Bing Ren

Detail(s)

Original languageEnglish
Pages (from-to)204-220
Journal / PublicationCell Research
Volume28
Issue number2
Online published9 Jan 2018
Publication statusPublished - Feb 2018
Externally publishedYes

Abstract

Long-range chromatin interactions between enhancers and promoters are essential for transcription of many developmentally controlled genes in mammals and other metazoans. Currently, the exact mechanisms that connect distal enhancers to their specific target promoters remain to be fully elucidated. Here, we show that the enhancer-specific histone H3 lysine 4 monomethylation (H3K4me1) and the histone methyltransferases MLL3 and MLL4 (MLL3/4) play an active role in this process. We demonstrate that in differentiating mouse embryonic stem cells, MLL3/4-dependent deposition of H3K4me1 at enhancers correlates with increased levels of chromatin interactions, whereas loss of this histone modification leads to reduced levels of chromatin interactions and defects in gene activation during differentiation. H3K4me1 facilitates recruitment of the Cohesin complex, a known regulator of chromatin organization, to chromatin in vitro and in vivo, providing a potential mechanism for MLL3/4 to promote chromatin interactions between enhancers and promoters. Taken together, our results support a role for MLL3/4-dependent H3K4me1 in orchestrating long-range chromatin interactions at enhancers in mammalian cells.

Research Area(s)

  • chromatin loop, Enhancer, H3K4me1, MLL3, MLL4

Citation Format(s)

Histone H3 lysine 4 monomethylation modulates long-range chromatin interactions at enhancers. / Yan, Jian; Chen, Shi-An A.; Local, Andrea; Liu, Tristin; Qiu, Yunjiang; Dorighi, Kristel M.; Preissl, Sebastian; Rivera, Chloe M.; Wang, Chaochen; Ye, Zhen; Ge, Kai; Hu, Ming; Wysocka, Joanna; Ren, Bing.

In: Cell Research, Vol. 28, No. 2, 02.2018, p. 204-220.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal