High-throughput microfluidic microarray hybridizations carried out in spiral channels on a compact disc

Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45)32_Refereed conference paper (with ISBN/ISSN)peer-review

1 Scopus Citations
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Author(s)

  • Hong Chen
  • Paul C.H. Li
  • Hua-Zhong Yu
  • M. Parameswaran
  • Nathan Yoganathan

Detail(s)

Original languageEnglish
Title of host publicationProceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
PublisherChemical and Biological Microsystems Society
Pages1282-1284
ISBN (Print)9780979806407
Publication statusPublished - 2007
Externally publishedYes

Publication series

NameProceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007

Conference

Title11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
PlaceFrance
CityParis
Period7 - 11 October 2007

Abstract

A high throughput microfluidic microarray assay (MMA) was developed through a two-step assembly process. First, a CD-like glass wafer was sealed with a PDMS chip with clockwise spiral channels to print probe lines onto the glass CD surface. Second, the glass wafer was sealed with another PDMS chip with anticlockwise spiral channels to allow the sample to hybridize with the printed probe lines. Using the MMA, 384 assays have been performed on CD at one time, using only 1-µL of each of the 96 samples. Here the hybridizations have been successfully achieved by using both complementary oligonucleotides and cRNA samples.

Research Area(s)

  • Dynamic DNA/RNA hybridization, Keyword: Microfluidic microarray, Multi-sample nucleic acid hybridization, Spiral microchannel

Bibliographic Note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to lbscholars@cityu.edu.hk.

Citation Format(s)

High-throughput microfluidic microarray hybridizations carried out in spiral channels on a compact disc. / Chen, Hong; Li, Paul C.H.; Yu, Hua-Zhong; Parameswaran, M.; Yoganathan, Nathan.

Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007. Chemical and Biological Microsystems Society, 2007. p. 1282-1284 (Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007).

Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45)32_Refereed conference paper (with ISBN/ISSN)peer-review