High-throughput DNA array for SNP detection of KRAS gene using a centrifugal microfluidic device

Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45)12_Chapter in an edited book (Author)peer-review

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Author(s)

Detail(s)

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages133-141
Volume1368
Publication statusPublished - 1 May 2016
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1368
ISSN (Print)1064-3745

Abstract

Here, we describe detection of single nucleotide polymorphism (SNP) in genomic DNA samples using a NanoBioArray (NBA) chip. Fast DNA hybridization is achieved in the chip when target DNAs are introduced to the surface-arrayed probes using centrifugal force. Gold nanoparticles (AuNPs) are used to assist SNP detection at room temperature. The parallel setting of sample introduction in the spiral channels of the NBA chip enables multiple analyses on many samples, resulting in a technique appropriate for highthroughput SNP detection. The experimental procedure, including chip fabrication, probe array printing, DNA amplification, hybridization, signal detection, and data analysis, is described in detail.

Research Area(s)

  • DNA microarray, Gold nanoparticles, Microfluidics, NanoBioArray (NBA), PDMS chip, Single nucleotide polymorphism (SNP)

Bibliographic Note

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Citation Format(s)

High-throughput DNA array for SNP detection of KRAS gene using a centrifugal microfluidic device. / Sedighi, Abootaleb; Li, Paul C. H.

Methods in Molecular Biology. Vol. 1368 Humana Press Inc., 2016. p. 133-141 (Methods in Molecular Biology; Vol. 1368).

Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45)12_Chapter in an edited book (Author)peer-review