High-speed label-free functional photoacoustic microscopy of mouse brain in action

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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Author(s)

  • Junjie Yao
  • Joon-Mo Yang
  • Konstantin I. Maslov
  • Terence T. W. Wong
  • Lei Li
  • Chih-Hsien Huang
  • Jun Zou
  • Lihong V. Wang

Detail(s)

Original languageEnglish
Pages (from-to)407-410
Journal / PublicationNature Methods
Volume12
Issue number5
Publication statusPublished - 29 Apr 2015
Externally publishedYes

Abstract

We present fast functional photoacoustic microscopy (PAM) for three-dimensional high-resolution, high-speed imaging of the mouse brain, complementary to other imaging modalities. We implemented a single-wavelength pulse-width-based method with a one-dimensional imaging rate of 100 kHz to image blood oxygenation with capillary-level resolution. We applied PAM to image the vascular morphology, blood oxygenation, blood flow and oxygen metabolism in both resting and stimulated states in the mouse brain.

Citation Format(s)

High-speed label-free functional photoacoustic microscopy of mouse brain in action. / Yao, Junjie; Wang, Lidai; Yang, Joon-Mo et al.
In: Nature Methods, Vol. 12, No. 5, 29.04.2015, p. 407-410.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review