High-speed label-free functional photoacoustic microscopy of mouse brain in action

Junjie Yao; Lidai Wang; Joon-Mo Yang; Konstantin I. Maslov; Terence T. W. Wong; Lei Li; Chih-Hsien Huang; Jun Zou; Lihong V. Wang

doi:10.1038/nmeth.3336

Author(s)

Junjie Yao
Joon-Mo Yang
Konstantin I. Maslov
Terence T. W. Wong
Lei Li
Chih-Hsien Huang
Jun Zou
Lihong V. Wang

Detail(s)

Original language	English
Pages (from-to)	407-410
Journal / Publication	Nature Methods
Volume	12
Issue number	5
Publication status	Published - 29 Apr 2015
Externally published	Yes

Link(s)

DOI	DOI https://doi.org/10.1038/nmeth.3336 Final Published version
	Check@CityULib
Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-84928926510&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(ffb1baa4-8e18-486c-be70-55ff50c5ee1a).html

Abstract

We present fast functional photoacoustic microscopy (PAM) for three-dimensional high-resolution, high-speed imaging of the mouse brain, complementary to other imaging modalities. We implemented a single-wavelength pulse-width-based method with a one-dimensional imaging rate of 100 kHz to image blood oxygenation with capillary-level resolution. We applied PAM to image the vascular morphology, blood oxygenation, blood flow and oxygen metabolism in both resting and stimulated states in the mouse brain.

Citation Format(s)

[ RIS ] [ BibTeX ]

High-speed label-free functional photoacoustic microscopy of mouse brain in action. / Yao, Junjie; Wang, Lidai; Yang, Joon-Mo et al.

In: Nature Methods, Vol. 12, No. 5, 29.04.2015, p. 407-410.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal › peer-review

Yao, J, Wang, L, Yang, J-M, Maslov, KI, Wong, TTW, Li, L, Huang, C-H, Zou, J & Wang, LV 2015, 'High-speed label-free functional photoacoustic microscopy of mouse brain in action', Nature Methods, vol. 12, no. 5, pp. 407-410. https://doi.org/10.1038/nmeth.3336

Yao, J., Wang, L., Yang, J-M., Maslov, K. I., Wong, T. T. W., Li, L., Huang, C-H., Zou, J., & Wang, L. V. (2015). High-speed label-free functional photoacoustic microscopy of mouse brain in action. Nature Methods, 12(5), 407-410. https://doi.org/10.1038/nmeth.3336

Yao J, Wang L, Yang J-M, Maslov KI, Wong TTW, Li L et al. High-speed label-free functional photoacoustic microscopy of mouse brain in action. Nature Methods. 2015 Apr 29;12(5):407-410. doi: 10.1038/nmeth.3336

Yao, Junjie ; Wang, Lidai ; Yang, Joon-Mo et al. / High-speed label-free functional photoacoustic microscopy of mouse brain in action. In: Nature Methods. 2015 ; Vol. 12, No. 5. pp. 407-410.