Highly Sensitive and Cost-Effective Portable Sensor for Early Gastric Carcinoma Diagnosis

Saw Lin Oo; Shishir Venkatesh; Vaithinathan Karthikeyan; Clement Manohar Arava; Spoorthy Pathikonda; Peter K. N. Yu; Terrence C. K. Lau; Xianfeng Chen; Vellaisamy A. L. Roy

doi:10.3390/s21082639

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Detail(s)

Original language	English
Article number	2639
Journal / Publication	Sensors
Volume	21
Issue number	8
Online published	9 Apr 2021
Publication status	Published - Apr 2021

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DOI	DOI https://doi.org/10.3390/s21082639 Final Published version
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Attachment(s)	Documents 74036251.pdf Final Published version, 5.81 MB, PDF Publisher's Copyright Statement This full text is made available under CC-BY 4.0. https://creativecommons.org/licenses/by/4.0/
Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-85103825846&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(f9967ffd-9643-4483-a397-d602121cf931).html

Abstract

Facile and efficient early detection of cancer is a major challenge in healthcare. Herein we developed a novel sensor made from a polycarbonate (PC) membrane with nanopores, followed by sequence-specific Oligo RNA modification for early gastric carcinoma diagnosis. In this design, the gastric cancer antigen CA72-4 is specifically conjugated to the Oligo RNA, thereby inhibiting the electrical current through the PC membrane in a concentration-dependent manner. The device can determine the concentration of cancer antigen CA72-4 in the range from 4 to 14 U/mL, possessing a sensitivity of 7.029 µAU⁻¹mLcm⁻² with a linear regression (R²) of 0.965 and a lower detection limit of 4 U/mL. This device has integrated advantages including high specificity and sensitivity and being simple, portable, and cost effective, which collectively enables a giant leap for cancer screening technologies towards clinical use. This is the first report to use RNA aptamers to detect CA72-4 for gastric carcinoma diagnosis.