Highly Sensitive and Cost-Effective Portable Sensor for Early Gastric Carcinoma Diagnosis

Saw Lin Oo; Shishir Venkatesh; Vaithinathan Karthikeyan; Clement Manohar Arava; Spoorthy Pathikonda; Peter K. N. Yu; Terrence C. K. Lau; Xianfeng Chen; Vellaisamy A. L. Roy

doi:10.3390/s21082639

Highly Sensitive and Cost-Effective Portable Sensor for Early Gastric Carcinoma Diagnosis

Saw Lin Oo, Shishir Venkatesh, Vaithinathan Karthikeyan, Clement Manohar Arava, Spoorthy Pathikonda, Peter K. N. Yu, Terrence C. K. Lau, Xianfeng Chen^*, Vellaisamy A. L. Roy^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

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Abstract

Facile and efficient early detection of cancer is a major challenge in healthcare. Herein we developed a novel sensor made from a polycarbonate (PC) membrane with nanopores, followed by sequence-specific Oligo RNA modification for early gastric carcinoma diagnosis. In this design, the gastric cancer antigen CA72-4 is specifically conjugated to the Oligo RNA, thereby inhibiting the electrical current through the PC membrane in a concentration-dependent manner. The device can determine the concentration of cancer antigen CA72-4 in the range from 4 to 14 U/mL, possessing a sensitivity of 7.029 µAU⁻¹mLcm⁻² with a linear regression (R²) of 0.965 and a lower detection limit of 4 U/mL. This device has integrated advantages including high specificity and sensitivity and being simple, portable, and cost effective, which collectively enables a giant leap for cancer screening technologies towards clinical use. This is the first report to use RNA aptamers to detect CA72-4 for gastric carcinoma diagnosis.

Original language	English
Article number	2639
Journal	Sensors
Volume	21
Issue number	8
Online published	9 Apr 2021
DOIs	https://doi.org/10.3390/s21082639
Publication status	Published - Apr 2021

Research Keywords

polycarbonate membrane
cancer antigen
Oligo RNA
cancer early detection
electrical current sensor

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This full text is made available under CC-BY 4.0. https://creativecommons.org/licenses/by/4.0/

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Highly Sensitive and Cost-Effective Portable Sensor for Early Gastric Carcinoma Diagnosis",

abstract = "Facile and efficient early detection of cancer is a major challenge in healthcare. Herein we developed a novel sensor made from a polycarbonate (PC) membrane with nanopores, followed by sequence-specific Oligo RNA modification for early gastric carcinoma diagnosis. In this design, the gastric cancer antigen CA72-4 is specifically conjugated to the Oligo RNA, thereby inhibiting the electrical current through the PC membrane in a concentration-dependent manner. The device can determine the concentration of cancer antigen CA72-4 in the range from 4 to 14 U/mL, possessing a sensitivity of 7.029 µAU−1mLcm−2 with a linear regression (R2) of 0.965 and a lower detection limit of 4 U/mL. This device has integrated advantages including high specificity and sensitivity and being simple, portable, and cost effective, which collectively enables a giant leap for cancer screening technologies towards clinical use. This is the first report to use RNA aptamers to detect CA72-4 for gastric carcinoma diagnosis.",

keywords = "polycarbonate membrane, cancer antigen, Oligo RNA, cancer early detection, electrical current sensor",

author = "Oo, {Saw Lin} and Shishir Venkatesh and Vaithinathan Karthikeyan and Arava, {Clement Manohar} and Spoorthy Pathikonda and Yu, {Peter K. N.} and Lau, {Terrence C. K.} and Xianfeng Chen and Roy, {Vellaisamy A. L.}",

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T1 - Highly Sensitive and Cost-Effective Portable Sensor for Early Gastric Carcinoma Diagnosis

AU - Oo, Saw Lin

AU - Venkatesh, Shishir

AU - Karthikeyan, Vaithinathan

AU - Arava, Clement Manohar

AU - Pathikonda, Spoorthy

AU - Yu, Peter K. N.

AU - Lau, Terrence C. K.

AU - Chen, Xianfeng

AU - Roy, Vellaisamy A. L.

PY - 2021/4

Y1 - 2021/4

N2 - Facile and efficient early detection of cancer is a major challenge in healthcare. Herein we developed a novel sensor made from a polycarbonate (PC) membrane with nanopores, followed by sequence-specific Oligo RNA modification for early gastric carcinoma diagnosis. In this design, the gastric cancer antigen CA72-4 is specifically conjugated to the Oligo RNA, thereby inhibiting the electrical current through the PC membrane in a concentration-dependent manner. The device can determine the concentration of cancer antigen CA72-4 in the range from 4 to 14 U/mL, possessing a sensitivity of 7.029 µAU−1mLcm−2 with a linear regression (R2) of 0.965 and a lower detection limit of 4 U/mL. This device has integrated advantages including high specificity and sensitivity and being simple, portable, and cost effective, which collectively enables a giant leap for cancer screening technologies towards clinical use. This is the first report to use RNA aptamers to detect CA72-4 for gastric carcinoma diagnosis.

AB - Facile and efficient early detection of cancer is a major challenge in healthcare. Herein we developed a novel sensor made from a polycarbonate (PC) membrane with nanopores, followed by sequence-specific Oligo RNA modification for early gastric carcinoma diagnosis. In this design, the gastric cancer antigen CA72-4 is specifically conjugated to the Oligo RNA, thereby inhibiting the electrical current through the PC membrane in a concentration-dependent manner. The device can determine the concentration of cancer antigen CA72-4 in the range from 4 to 14 U/mL, possessing a sensitivity of 7.029 µAU−1mLcm−2 with a linear regression (R2) of 0.965 and a lower detection limit of 4 U/mL. This device has integrated advantages including high specificity and sensitivity and being simple, portable, and cost effective, which collectively enables a giant leap for cancer screening technologies towards clinical use. This is the first report to use RNA aptamers to detect CA72-4 for gastric carcinoma diagnosis.

KW - polycarbonate membrane

KW - cancer antigen

KW - Oligo RNA

KW - cancer early detection

KW - electrical current sensor

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DO - 10.3390/s21082639

M3 - RGC 21 - Publication in refereed journal

C2 - 33918707

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JF - Sensors

IS - 8

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ER -

Highly Sensitive and Cost-Effective Portable Sensor for Early Gastric Carcinoma Diagnosis

Abstract

Research Keywords

Publisher's Copyright Statement

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