Highly efficient transgenesis with miniMos in Caenorhabditis briggsae

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

1 Scopus Citations
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Author(s)

  • Qiutao Ding
  • Xiaoliang Ren
  • Luyan Chan
  • Vincy W. S. Ho
  • Yu Bi
  • Dongying Xie
  • Zhongying Zhao

Detail(s)

Original languageEnglish
Article numberjkac254
Journal / PublicationG3: Genes, Genomes, Genetics
Volume12
Issue number12
Online published28 Sept 2022
Publication statusPublished - Dec 2022

Link(s)

Abstract

Caenorhabditis briggsae as a companion species for Caenorhabditis elegans has played an increasingly important role in study of evolution of development and genome and gene regulation. Aided by the isolation of its sister spices, it has recently been established as a model for speciation study. To take full advantage of the species for comparative study, an effective transgenesis method especially those with single-copy insertion is important for functional comparison. Here, we improved a transposon-based transgenesis methodology that had been originally developed in C. elegans but worked marginally in C. briggsae. By incorporation of a heat shock step, the transgenesis efficiency in C. briggsae with a single-copy insertion is comparable to that in C. elegans. We used the method to generate 54 independent insertions mostly consisting of a mCherry tag over the C. briggsae genome. We demonstrated the use of the tags in identifying interacting loci responsible for hybrid male sterility between C. briggsae and Caenorhabditis nigoni when combined with the GFP tags we generated previously. Finally, we demonstrated that C. briggsae tolerates the C. elegans toxin, PEEL-1, but not SUP-35, making the latter a potential negative selection marker against extrachromosomal array.

Research Area(s)

  • miniMos, Caenorhabditis briggsae, heat shock, transgenesis, C. ELEGANS, GENOME, SELECTION, TRANSPOSONS, EXPRESSION, INSERTION, INSIGHTS, BIOLOGY, STRESS, HYBRID

Citation Format(s)

Highly efficient transgenesis with miniMos in Caenorhabditis briggsae. / Ding, Qiutao; Ren, Xiaoliang; Li, Runsheng et al.
In: G3: Genes, Genomes, Genetics, Vol. 12, No. 12, jkac254, 12.2022.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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