High-efficiency quantitative control of mitochondrial transfer based on droplet microfluidics and its application on muscle regeneration

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

15 Scopus Citations
View graph of relations

Author(s)

  • Hiu Tung Jessica Lo
  • Tsz Lam Yiu
  • Gang Li
  • Wayne Y. W. Lee

Detail(s)

Original languageEnglish
Article numbereabp9245
Journal / PublicationScience Advances
Volume8
Issue number33
Online published17 Aug 2022
Publication statusPublished - 19 Aug 2022

Link(s)

Abstract

Mitochondrial transfer is a spontaneous process to restore damaged cells in various pathological conditions. The transfer of mitochondria to cell therapy products before their administration can enhance therapeutic outcomes. However, the low efficiency of previously reported methods limits their clinical application. Here, we developed a droplet microfluidics-based mitochondrial transfer technique that can achieve high-efficiency and high-throughput quantitative mitochondrial transfer to single cells. Because mitochondria are essential for muscles, myoblast cells and a muscle injury model were used as a proof-of-concept model to evaluate the proposed technique. In vitro and in vivo experiments demonstrated that C2C12 cells with 31 transferred mitochondria had significant improvements in cellular functions compared to those with 0, 8, and 14 transferred mitochondria and also had better therapeutic effects on muscle regeneration. The proposed technique can considerably promote the clinical application of mitochondrial transfer, with optimized cell function improvements, for the cell therapy of mitochondria-related diseases.

Research Area(s)

Citation Format(s)

Download Statistics

No data available