TY - GEN
T1 - High-throughput zebrafish immobilization by a fish-shaped microfluidic device for behavioral study
AU - Chen, W.
AU - Chen, J.
AU - Wen, C.
AU - Walker, S.
AU - Ke, Y.
AU - Yung, W.
AU - Chen, S. C.
N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].
PY - 2016
Y1 - 2016
N2 - This paper reports a high-throughput microfluidic platform that enables long-term monitoring of unanesthetized zebrafish larva behavioral responses, i.e., heart rate and fin flapping frequency, under different electric stimulations. In the device, since the body of the zebrafish are well constrained by the fish-shaped chambers, only a small pressure drop (< 500Pa) is required for stable immobilization. Experimental results show our device can effectively constrain the zebrafish with a 100% 5-day survival rate without altering normal physiological state. For behavioral studies, interdigitated microelectrodes are integrated beneath the brain of zebrafish to deliver electric stimuli. Corresponding physiological states and Ca2+ imaging of neuron-firings in GaCamp6 labeled zebrafish have been successfully recorded in real-time via our custom-designed two-photon microscope.
AB - This paper reports a high-throughput microfluidic platform that enables long-term monitoring of unanesthetized zebrafish larva behavioral responses, i.e., heart rate and fin flapping frequency, under different electric stimulations. In the device, since the body of the zebrafish are well constrained by the fish-shaped chambers, only a small pressure drop (< 500Pa) is required for stable immobilization. Experimental results show our device can effectively constrain the zebrafish with a 100% 5-day survival rate without altering normal physiological state. For behavioral studies, interdigitated microelectrodes are integrated beneath the brain of zebrafish to deliver electric stimuli. Corresponding physiological states and Ca2+ imaging of neuron-firings in GaCamp6 labeled zebrafish have been successfully recorded in real-time via our custom-designed two-photon microscope.
KW - Behavioral study
KW - Immobilization
KW - Microfluidic device
KW - Zebrafish larva
UR - http://www.scopus.com/inward/record.url?scp=85014163555&partnerID=8YFLogxK
UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85014163555&origin=recordpage
M3 - RGC 32 - Refereed conference paper (with host publication)
SN - 9780979806490
T3 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
SP - 469
EP - 470
BT - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PB - Chemical and Biological Microsystems Society
T2 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Y2 - 9 October 2016 through 13 October 2016
ER -