HIGH-THROUGHPUT SINGLE CELL PROTEASE ANALYSIS ON HUMAN CIRCULATING TUMOR CELLS

Tengyang Jing; Ee Xien Ng; Bee Luan Khoo; Chwee Teck Lim; Jongyoon Han; Chia-Hung Chen

HIGH-THROUGHPUT SINGLE CELL PROTEASE ANALYSIS ON HUMAN CIRCULATING TUMOR CELLS

Tengyang Jing
, Ee Xien Ng
, Bee Luan Khoo
, Chwee Teck Lim
, Jongyoon Han^*
, Chia-Hung Chen

^*Corresponding author for this work

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

Abstract

Detection of circulating tumor cells (CTCs) from cancer patients' blood provides a convenient method for early-stage cancer diagnosis and to monitor disease progression. Many studies have confirmed that tumor consists of a heterogeneous cell composition. Only a small portion of CTCs can persist within the blood circulatory system. Residing CTCs may then extravasate to form a secondary tumor, mediated by the secretion of matrix metalloproteinases (MMP). Here, we present an integrated microfluidic approach for MMP activity measurement on sorted single CTCs. The assay results could contribute to identify CTC subpopulations of different metastatic potential and provide insights into cancer heterogeneity.

Original language	English
Title of host publication	19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015)
Publisher	Chemical and Biological Microsystems Society
Pages	490-492
Volume	1
ISBN (Electronic)	9780979806483
ISBN (Print)	9781510817876
Publication status	Published - Oct 2015
Externally published	Yes
Event	19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015) - Gyeongju, Korea, Republic of Duration: 25 Oct 2015 → 29 Oct 2015

Conference

Conference	19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015)
Abbreviated title	MicroTAS 2015
Place	Korea, Republic of
City	Gyeongju
Period	25/10/15 → 29/10/15

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Research Keywords

Circulating tumor cell
Matrix metalloproteinases functional assay
Single cell analysis

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@inproceedings{13c3a4c86fe2447ea99aba68826bb70d,

title = "HIGH-THROUGHPUT SINGLE CELL PROTEASE ANALYSIS ON HUMAN CIRCULATING TUMOR CELLS",

abstract = "Detection of circulating tumor cells (CTCs) from cancer patients' blood provides a convenient method for early-stage cancer diagnosis and to monitor disease progression. Many studies have confirmed that tumor consists of a heterogeneous cell composition. Only a small portion of CTCs can persist within the blood circulatory system. Residing CTCs may then extravasate to form a secondary tumor, mediated by the secretion of matrix metalloproteinases (MMP). Here, we present an integrated microfluidic approach for MMP activity measurement on sorted single CTCs. The assay results could contribute to identify CTC subpopulations of different metastatic potential and provide insights into cancer heterogeneity.",

keywords = "Circulating tumor cell, Matrix metalloproteinases functional assay, Single cell analysis",

author = "Tengyang Jing and Ng, \{Ee Xien\} and Khoo, \{Bee Luan\} and Lim, \{Chwee Teck\} and Jongyoon Han and Chia-Hung Chen",

year = "2015",

month = oct,

language = "English",

isbn = "9781510817876",

volume = "1",

pages = "490--492",

booktitle = "19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015)",

publisher = "Chemical and Biological Microsystems Society",

note = "19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015), MicroTAS 2015 ; Conference date: 25-10-2015 Through 29-10-2015",

}

Jing, T, Ng, EX, Khoo, BL, Lim, CT, Han, J & Chen, C-H 2015, HIGH-THROUGHPUT SINGLE CELL PROTEASE ANALYSIS ON HUMAN CIRCULATING TUMOR CELLS. in 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015). vol. 1, Chemical and Biological Microsystems Society, pp. 490-492, 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015), Gyeongju, Korea, Republic of, 25/10/15.

HIGH-THROUGHPUT SINGLE CELL PROTEASE ANALYSIS ON HUMAN CIRCULATING TUMOR CELLS. / Jing, Tengyang; Ng, Ee Xien; Khoo, Bee Luan et al.
19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015). Vol. 1 Chemical and Biological Microsystems Society, 2015. p. 490-492.

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - HIGH-THROUGHPUT SINGLE CELL PROTEASE ANALYSIS ON HUMAN CIRCULATING TUMOR CELLS

AU - Jing, Tengyang

AU - Ng, Ee Xien

AU - Khoo, Bee Luan

AU - Lim, Chwee Teck

AU - Han, Jongyoon

AU - Chen, Chia-Hung

PY - 2015/10

Y1 - 2015/10

N2 - Detection of circulating tumor cells (CTCs) from cancer patients' blood provides a convenient method for early-stage cancer diagnosis and to monitor disease progression. Many studies have confirmed that tumor consists of a heterogeneous cell composition. Only a small portion of CTCs can persist within the blood circulatory system. Residing CTCs may then extravasate to form a secondary tumor, mediated by the secretion of matrix metalloproteinases (MMP). Here, we present an integrated microfluidic approach for MMP activity measurement on sorted single CTCs. The assay results could contribute to identify CTC subpopulations of different metastatic potential and provide insights into cancer heterogeneity.

AB - Detection of circulating tumor cells (CTCs) from cancer patients' blood provides a convenient method for early-stage cancer diagnosis and to monitor disease progression. Many studies have confirmed that tumor consists of a heterogeneous cell composition. Only a small portion of CTCs can persist within the blood circulatory system. Residing CTCs may then extravasate to form a secondary tumor, mediated by the secretion of matrix metalloproteinases (MMP). Here, we present an integrated microfluidic approach for MMP activity measurement on sorted single CTCs. The assay results could contribute to identify CTC subpopulations of different metastatic potential and provide insights into cancer heterogeneity.

KW - Circulating tumor cell

KW - Matrix metalloproteinases functional assay

KW - Single cell analysis

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M3 - RGC 32 - Refereed conference paper (with host publication)

SN - 9781510817876

VL - 1

SP - 490

EP - 492

BT - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015)

PB - Chemical and Biological Microsystems Society

T2 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015)

Y2 - 25 October 2015 through 29 October 2015

ER -

HIGH-THROUGHPUT SINGLE CELL PROTEASE ANALYSIS ON HUMAN CIRCULATING TUMOR CELLS

Abstract

Conference

UN SDGs

Research Keywords

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