High-Throughput Plasmonic Single Cell Immunoassay

Shih-Chung Wei; Myat Noe Hsu; Wei-Chuan Shih; Chia-Hung Chen

High-Throughput Plasmonic Single Cell Immunoassay

Shih-Chung Wei
, Myat Noe Hsu
, Wei-Chuan Shih
, Chia-Hung Chen^*

^*Corresponding author for this work

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

Abstract

A one-step plasmonic droplet immunoassay was developed for high-throughput and label-free single-cell secretomic analysis. With the characteristic of nanogold sensor, this is a truly non-wash assay in droplet for single cell secretion detection. The detection range of this plasmonic assay in bulk is from 500 pg/ml to 50 ng/ml. In droplet, the plasmonic assay is also sensitive to secretory IL-8 in a concentration of 1 ng/ml. We demonstrate the possibility of using this assay to identify different phenotype of MDA-MB-231 and HL-60 cell line in this work. This assay has shown its potential in single-cell phenotype screening for further applications.

Original language	English
Title of host publication	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018)
Publisher	Chemical and Biological Microsystems Society
Pages	1357-1359
Volume	3
ISBN (Electronic)	9780578405308
ISBN (Print)	9781510897571
Publication status	Published - Nov 2018
Externally published	Yes
Event	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018) - Kaohsiung Exhibition Center, Kaohsiung, Taiwan, China Duration: 11 Nov 2018 → 15 Nov 2018 https://cbmsociety.org/microtas/microtas2018/ https://cbmsociety.org/wp-content/uploads/2018/11/uTAS-2018-FInal-Program-WEB.pdf https://archive.cbmsociety.org/utas/uTAS2018.zip

Publication series

Name	International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS
Publisher	Chemical and Biological Microsystems Society
ISSN (Electronic)	1556-5904

Conference

Conference	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018)
Abbreviated title	μTAS2018
Place	Taiwan, China
City	Kaohsiung
Period	11/11/18 → 15/11/18
Internet address	https://cbmsociety.org/microtas/microtas2018/ https://cbmsociety.org/wp-content/uploads/2018/11/uTAS-2018-FInal-Program-WEB.pdf https://archive.cbmsociety.org/utas/uTAS2018.zip

Research Keywords

Droplet
Immunoassay
Plasmonic
Secretion
Single-cell

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Wei, S.-C., Hsu, M. N., Shih, W.-C., & Chen, C.-H. (2018). High-Throughput Plasmonic Single Cell Immunoassay. In 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018) (Vol. 3, pp. 1357-1359). (International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS). Chemical and Biological Microsystems Society.

Wei, Shih-Chung ; Hsu, Myat Noe ; Shih, Wei-Chuan et al. / High-Throughput Plasmonic Single Cell Immunoassay. 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018). Vol. 3 Chemical and Biological Microsystems Society, 2018. pp. 1357-1359 (International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS).

@inproceedings{f16fdd5974874fd5bdf978f462faf2b8,

title = "High-Throughput Plasmonic Single Cell Immunoassay",

abstract = "A one-step plasmonic droplet immunoassay was developed for high-throughput and label-free single-cell secretomic analysis. With the characteristic of nanogold sensor, this is a truly non-wash assay in droplet for single cell secretion detection. The detection range of this plasmonic assay in bulk is from 500 pg/ml to 50 ng/ml. In droplet, the plasmonic assay is also sensitive to secretory IL-8 in a concentration of 1 ng/ml. We demonstrate the possibility of using this assay to identify different phenotype of MDA-MB-231 and HL-60 cell line in this work. This assay has shown its potential in single-cell phenotype screening for further applications.",

keywords = "Droplet, Immunoassay, Plasmonic, Secretion, Single-cell",

author = "Shih-Chung Wei and Hsu, \{Myat Noe\} and Wei-Chuan Shih and Chia-Hung Chen",

year = "2018",

month = nov,

language = "English",

isbn = "9781510897571",

volume = "3",

series = "International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS",

publisher = "Chemical and Biological Microsystems Society",

pages = "1357--1359",

booktitle = "22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018)",

note = "22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018), μTAS2018 ; Conference date: 11-11-2018 Through 15-11-2018",

url = "https://cbmsociety.org/microtas/microtas2018/, https://cbmsociety.org/wp-content/uploads/2018/11/uTAS-2018-FInal-Program-WEB.pdf, https://archive.cbmsociety.org/utas/uTAS2018.zip",

}

Wei, S-C, Hsu, MN, Shih, W-C & Chen, C-H 2018, High-Throughput Plasmonic Single Cell Immunoassay. in 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018). vol. 3, International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS, Chemical and Biological Microsystems Society, pp. 1357-1359, 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018), Kaohsiung, Taiwan, China, 11/11/18.

High-Throughput Plasmonic Single Cell Immunoassay. / Wei, Shih-Chung; Hsu, Myat Noe; Shih, Wei-Chuan et al.
22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018). Vol. 3 Chemical and Biological Microsystems Society, 2018. p. 1357-1359 (International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS).

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - High-Throughput Plasmonic Single Cell Immunoassay

AU - Wei, Shih-Chung

AU - Hsu, Myat Noe

AU - Shih, Wei-Chuan

AU - Chen, Chia-Hung

PY - 2018/11

Y1 - 2018/11

N2 - A one-step plasmonic droplet immunoassay was developed for high-throughput and label-free single-cell secretomic analysis. With the characteristic of nanogold sensor, this is a truly non-wash assay in droplet for single cell secretion detection. The detection range of this plasmonic assay in bulk is from 500 pg/ml to 50 ng/ml. In droplet, the plasmonic assay is also sensitive to secretory IL-8 in a concentration of 1 ng/ml. We demonstrate the possibility of using this assay to identify different phenotype of MDA-MB-231 and HL-60 cell line in this work. This assay has shown its potential in single-cell phenotype screening for further applications.

AB - A one-step plasmonic droplet immunoassay was developed for high-throughput and label-free single-cell secretomic analysis. With the characteristic of nanogold sensor, this is a truly non-wash assay in droplet for single cell secretion detection. The detection range of this plasmonic assay in bulk is from 500 pg/ml to 50 ng/ml. In droplet, the plasmonic assay is also sensitive to secretory IL-8 in a concentration of 1 ng/ml. We demonstrate the possibility of using this assay to identify different phenotype of MDA-MB-231 and HL-60 cell line in this work. This assay has shown its potential in single-cell phenotype screening for further applications.

KW - Droplet

KW - Immunoassay

KW - Plasmonic

KW - Secretion

KW - Single-cell

UR - https://www.scopus.com/pages/publications/85079814936

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85079814936&origin=recordpage

M3 - RGC 32 - Refereed conference paper (with host publication)

SN - 9781510897571

VL - 3

T3 - International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS

SP - 1357

EP - 1359

BT - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018)

PB - Chemical and Biological Microsystems Society

T2 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018)

Y2 - 11 November 2018 through 15 November 2018

ER -

High-Throughput Plasmonic Single Cell Immunoassay

Abstract

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Research Keywords

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