@inbook{c3b01b0b3441439f838d5b9f5b80f68c,
title = "High-throughput DNA array for SNP detection of KRAS gene using a centrifugal microfluidic device",
abstract = "Here, we describe detection of single nucleotide polymorphism (SNP) in genomic DNA samples using a NanoBioArray (NBA) chip. Fast DNA hybridization is achieved in the chip when target DNAs are introduced to the surface-arrayed probes using centrifugal force. Gold nanoparticles (AuNPs) are used to assist SNP detection at room temperature. The parallel setting of sample introduction in the spiral channels of the NBA chip enables multiple analyses on many samples, resulting in a technique appropriate for highthroughput SNP detection. The experimental procedure, including chip fabrication, probe array printing, DNA amplification, hybridization, signal detection, and data analysis, is described in detail.",
keywords = "DNA microarray, Gold nanoparticles, Microfluidics, NanoBioArray (NBA), PDMS chip, Single nucleotide polymorphism (SNP), DNA microarray, Gold nanoparticles, Microfluidics, NanoBioArray (NBA), PDMS chip, Single nucleotide polymorphism (SNP), DNA microarray, Gold nanoparticles, Microfluidics, NanoBioArray (NBA), PDMS chip, Single nucleotide polymorphism (SNP)",
author = "Abootaleb Sedighi and Li, \{Paul C. H.\}",
note = "Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to
[email protected].",
year = "2016",
month = may,
day = "1",
doi = "10.1007/978-1-4939-3136-1\_10",
language = "English",
volume = "1368",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "133--141",
booktitle = "Methods in Molecular Biology",
}