HIGH THROUGHPUT CIRCULATING TUMOR CELL ISOLATION USING TRAPEZOIDAL INERTIAL MICROFLUIDICS

Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45)32_Refereed conference paper (with ISBN/ISSN)peer-review

4 Scopus Citations
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Author(s)

  • G. Guan
  • M.E. Warkiani
  • C.T. Lim
  • P.C.Y. Chen
  • J. Han

Detail(s)

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013)
PublisherChemical and Biological Microsystems Society
Pages23-25
Volume1
ISBN (Print)9781632666246
Publication statusPublished - Oct 2013
Externally publishedYes

Conference

Title17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013)
LocationMesse Freiburg
PlaceGermany
CityFreiburg
Period27 - 31 October 2013

Abstract

A novel Dean coupled inertial microfluidic device with trapezoidal cross-section spiral channel for ultra-fast, label- free enrichment of circulating tumor cells (CTCs) from clinically relevant blood volumes is reported in this work. Using this single spiral microchannel with one inlet and two outlets, we have successfully isolated and recovered more than 80% of cancer cell line cells spiked in 7.5 mL of blood within 8 min with high purity. Putative CTCs were detected and isolated from 100% patient samples with advanced stage metastatic breast and lung cancer using standard biomarkers. DNA fluorescence in-situ hybridization (FISH) was also carried out to evaluate HER2 status in CTCs isolated from patient samples. Copyright © (2013) by the Chemical and Biological Microsystems Society All rights reserved.

Research Area(s)

  • Circulating tumor cells, Inertial microfluidics, Size based separation, Trapezoidal cross-section

Citation Format(s)

HIGH THROUGHPUT CIRCULATING TUMOR CELL ISOLATION USING TRAPEZOIDAL INERTIAL MICROFLUIDICS. / Guan, G.; Warkiani, M.E.; Luan, K.B. et al.

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013) . Vol. 1 Chemical and Biological Microsystems Society, 2013. p. 23-25.

Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45)32_Refereed conference paper (with ISBN/ISSN)peer-review