HIGH THROUGHPUT CIRCULATING TUMOR CELL ISOLATION USING TRAPEZOIDAL INERTIAL MICROFLUIDICS

G. Guan; M.E. Warkiani; K.B. Luan; C.T. Lim; P.C.Y. Chen; J. Han

HIGH THROUGHPUT CIRCULATING TUMOR CELL ISOLATION USING TRAPEZOIDAL INERTIAL MICROFLUIDICS

G. Guan
, M.E. Warkiani
, K.B. Luan
, C.T. Lim
, P.C.Y. Chen
, J. Han

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

5 Citations (Scopus)

Abstract

A novel Dean coupled inertial microfluidic device with trapezoidal cross-section spiral channel for ultra-fast, label- free enrichment of circulating tumor cells (CTCs) from clinically relevant blood volumes is reported in this work. Using this single spiral microchannel with one inlet and two outlets, we have successfully isolated and recovered more than 80% of cancer cell line cells spiked in 7.5 mL of blood within 8 min with high purity. Putative CTCs were detected and isolated from 100% patient samples with advanced stage metastatic breast and lung cancer using standard biomarkers. DNA fluorescence in-situ hybridization (FISH) was also carried out to evaluate HER2 status in CTCs isolated from patient samples. Copyright © (2013) by the Chemical and Biological Microsystems Society All rights reserved.

Original language	English
Title of host publication	17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013)
Publisher	Chemical and Biological Microsystems Society
Pages	23-25
Volume	1
ISBN (Print)	9781632666246
Publication status	Published - Oct 2013
Externally published	Yes
Event	17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013) - Messe Freiburg, Freiburg, Germany Duration: 27 Oct 2013 → 31 Oct 2013

Conference

Conference	17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013)
Place	Germany
City	Freiburg
Period	27/10/13 → 31/10/13

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Research Keywords

Circulating tumor cells
Inertial microfluidics
Size based separation
Trapezoidal cross-section

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@inproceedings{fe79c22a1bdd468fa0a717aeb8129404,

title = "HIGH THROUGHPUT CIRCULATING TUMOR CELL ISOLATION USING TRAPEZOIDAL INERTIAL MICROFLUIDICS",

abstract = "A novel Dean coupled inertial microfluidic device with trapezoidal cross-section spiral channel for ultra-fast, label- free enrichment of circulating tumor cells (CTCs) from clinically relevant blood volumes is reported in this work. Using this single spiral microchannel with one inlet and two outlets, we have successfully isolated and recovered more than 80\% of cancer cell line cells spiked in 7.5 mL of blood within 8 min with high purity. Putative CTCs were detected and isolated from 100\% patient samples with advanced stage metastatic breast and lung cancer using standard biomarkers. DNA fluorescence in-situ hybridization (FISH) was also carried out to evaluate HER2 status in CTCs isolated from patient samples. Copyright {\textcopyright} (2013) by the Chemical and Biological Microsystems Society All rights reserved.",

keywords = "Circulating tumor cells, Inertial microfluidics, Size based separation, Trapezoidal cross-section",

author = "G. Guan and M.E. Warkiani and K.B. Luan and C.T. Lim and P.C.Y. Chen and J. Han",

year = "2013",

month = oct,

language = "English",

isbn = "9781632666246",

volume = "1",

pages = "23--25",

booktitle = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013)",

publisher = "Chemical and Biological Microsystems Society",

note = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013) ; Conference date: 27-10-2013 Through 31-10-2013",

}

Guan, G, Warkiani, ME, Luan, KB, Lim, CT, Chen, PCY & Han, J 2013, HIGH THROUGHPUT CIRCULATING TUMOR CELL ISOLATION USING TRAPEZOIDAL INERTIAL MICROFLUIDICS. in 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013) . vol. 1, Chemical and Biological Microsystems Society, pp. 23-25, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013), Freiburg, Germany, 27/10/13.

HIGH THROUGHPUT CIRCULATING TUMOR CELL ISOLATION USING TRAPEZOIDAL INERTIAL MICROFLUIDICS. / Guan, G.; Warkiani, M.E.; Luan, K.B. et al.
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013) . Vol. 1 Chemical and Biological Microsystems Society, 2013. p. 23-25.

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - HIGH THROUGHPUT CIRCULATING TUMOR CELL ISOLATION USING TRAPEZOIDAL INERTIAL MICROFLUIDICS

AU - Guan, G.

AU - Warkiani, M.E.

AU - Luan, K.B.

AU - Lim, C.T.

AU - Chen, P.C.Y.

AU - Han, J.

PY - 2013/10

Y1 - 2013/10

N2 - A novel Dean coupled inertial microfluidic device with trapezoidal cross-section spiral channel for ultra-fast, label- free enrichment of circulating tumor cells (CTCs) from clinically relevant blood volumes is reported in this work. Using this single spiral microchannel with one inlet and two outlets, we have successfully isolated and recovered more than 80% of cancer cell line cells spiked in 7.5 mL of blood within 8 min with high purity. Putative CTCs were detected and isolated from 100% patient samples with advanced stage metastatic breast and lung cancer using standard biomarkers. DNA fluorescence in-situ hybridization (FISH) was also carried out to evaluate HER2 status in CTCs isolated from patient samples. Copyright © (2013) by the Chemical and Biological Microsystems Society All rights reserved.

AB - A novel Dean coupled inertial microfluidic device with trapezoidal cross-section spiral channel for ultra-fast, label- free enrichment of circulating tumor cells (CTCs) from clinically relevant blood volumes is reported in this work. Using this single spiral microchannel with one inlet and two outlets, we have successfully isolated and recovered more than 80% of cancer cell line cells spiked in 7.5 mL of blood within 8 min with high purity. Putative CTCs were detected and isolated from 100% patient samples with advanced stage metastatic breast and lung cancer using standard biomarkers. DNA fluorescence in-situ hybridization (FISH) was also carried out to evaluate HER2 status in CTCs isolated from patient samples. Copyright © (2013) by the Chemical and Biological Microsystems Society All rights reserved.

KW - Circulating tumor cells

KW - Inertial microfluidics

KW - Size based separation

KW - Trapezoidal cross-section

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UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-84904382083&origin=recordpage

M3 - RGC 32 - Refereed conference paper (with host publication)

SN - 9781632666246

VL - 1

SP - 23

EP - 25

BT - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013)

PB - Chemical and Biological Microsystems Society

T2 - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013)

Y2 - 27 October 2013 through 31 October 2013

ER -

HIGH THROUGHPUT CIRCULATING TUMOR CELL ISOLATION USING TRAPEZOIDAL INERTIAL MICROFLUIDICS

Abstract

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UN SDGs

Research Keywords

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