Heteroleptic Ruthenium(II) Complexes with Bathophenanthroline and Bathophenanthroline Disulfonate Disodium Salt as Fluorescent Dyes for In-Gel Protein Staining

Maria V. Babak; Pauline Le Faouder; Xavier Trivelli; Gopalakrishnan Venkatesan; Stanislav I. Bezzubov; Mohammed Kajjout; Artem L. Gushchin; Muhammad Hanif; Olivier Poizat; Hervé Vezin; Christian Rolando

doi:10.1021/acs.inorgchem.9b03679

Heteroleptic Ruthenium(II) Complexes with Bathophenanthroline and Bathophenanthroline Disulfonate Disodium Salt as Fluorescent Dyes for In-Gel Protein Staining

Maria V. Babak^*
, Pauline Le Faouder
, Xavier Trivelli
, Gopalakrishnan Venkatesan
, Stanislav I. Bezzubov
, Mohammed Kajjout
, Artem L. Gushchin
, Muhammad Hanif
, Olivier Poizat
, Hervé Vezin
, Christian Rolando

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

10 Citations (Scopus)

Abstract

The in-gel detection of proteins for various proteomic experiments is commonly done with the fluorescent Ru^II tris(bathophenanthroline disulfonate) complex (Ru(BPS)₃), which is more cost-effective compared to commercial Ru-based formulations but requires tedious procedures for its preparation and strongly acidic staining conditions. Herein, we report the synthesis and characterization of heteroleptic Ru^II complexes Ru(BPS)₂(BP) and Ru(BPS)(BP)₂ containing bathophenanthroline (BP) and bathophenanthroline disulfonate disodium salt (BPS) in comparison with Ru(BPS)₃. It was shown by fluorescent and UV-vis measurements that novel Ru^II complexes were excitable in both UV and visible light, close to emission bands of classical lasers, which is important for successful in-gel protein detection. Novel fluorescent dyes demonstrated improved protein detection in comparison with commercially available SYPRO Ruby staining solution. In addition, unlike commonly used staining protocols, staining with Ru(BPS)(BP)₂ can be performed at nearly neutral pH, thereby reducing artificial post-translational modifications (PTMs).

Original language	English
Pages (from-to)	4527-4535
Journal	Inorganic Chemistry
Volume	59
Issue number	7
Online published	17 Mar 2020
DOIs	https://doi.org/10.1021/acs.inorgchem.9b03679
Publication status	Published - 6 Apr 2020
Externally published	Yes

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Babak, M. V., Le Faouder, P., Trivelli, X., Venkatesan, G., Bezzubov, S. I., Kajjout, M., Gushchin, A. L., Hanif, M., Poizat, O., Vezin, H., & Rolando, C. (2020). Heteroleptic Ruthenium(II) Complexes with Bathophenanthroline and Bathophenanthroline Disulfonate Disodium Salt as Fluorescent Dyes for In-Gel Protein Staining. Inorganic Chemistry, 59(7), 4527-4535. https://doi.org/10.1021/acs.inorgchem.9b03679

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title = "Heteroleptic Ruthenium(II) Complexes with Bathophenanthroline and Bathophenanthroline Disulfonate Disodium Salt as Fluorescent Dyes for In-Gel Protein Staining",

abstract = "The in-gel detection of proteins for various proteomic experiments is commonly done with the fluorescent RuII tris(bathophenanthroline disulfonate) complex (Ru(BPS)3), which is more cost-effective compared to commercial Ru-based formulations but requires tedious procedures for its preparation and strongly acidic staining conditions. Herein, we report the synthesis and characterization of heteroleptic RuII complexes Ru(BPS)2(BP) and Ru(BPS)(BP)2 containing bathophenanthroline (BP) and bathophenanthroline disulfonate disodium salt (BPS) in comparison with Ru(BPS)3. It was shown by fluorescent and UV-vis measurements that novel RuII complexes were excitable in both UV and visible light, close to emission bands of classical lasers, which is important for successful in-gel protein detection. Novel fluorescent dyes demonstrated improved protein detection in comparison with commercially available SYPRO Ruby staining solution. In addition, unlike commonly used staining protocols, staining with Ru(BPS)(BP)2 can be performed at nearly neutral pH, thereby reducing artificial post-translational modifications (PTMs).",

author = "Babak, \{Maria V.\} and \{Le Faouder\}, Pauline and Xavier Trivelli and Gopalakrishnan Venkatesan and Bezzubov, \{Stanislav I.\} and Mohammed Kajjout and Gushchin, \{Artem L.\} and Muhammad Hanif and Olivier Poizat and Herv{\'e} Vezin and Christian Rolando",

year = "2020",

month = apr,

day = "6",

doi = "10.1021/acs.inorgchem.9b03679",

language = "English",

volume = "59",

pages = "4527--4535",

journal = "Inorganic Chemistry",

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Babak, MV, Le Faouder, P, Trivelli, X, Venkatesan, G, Bezzubov, SI, Kajjout, M, Gushchin, AL, Hanif, M, Poizat, O, Vezin, H & Rolando, C 2020, 'Heteroleptic Ruthenium(II) Complexes with Bathophenanthroline and Bathophenanthroline Disulfonate Disodium Salt as Fluorescent Dyes for In-Gel Protein Staining', Inorganic Chemistry, vol. 59, no. 7, pp. 4527-4535. https://doi.org/10.1021/acs.inorgchem.9b03679

Heteroleptic Ruthenium(II) Complexes with Bathophenanthroline and Bathophenanthroline Disulfonate Disodium Salt as Fluorescent Dyes for In-Gel Protein Staining. / Babak, Maria V.; Le Faouder, Pauline; Trivelli, Xavier et al.
In: Inorganic Chemistry, Vol. 59, No. 7, 06.04.2020, p. 4527-4535.

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

TY - JOUR

T1 - Heteroleptic Ruthenium(II) Complexes with Bathophenanthroline and Bathophenanthroline Disulfonate Disodium Salt as Fluorescent Dyes for In-Gel Protein Staining

AU - Babak, Maria V.

AU - Le Faouder, Pauline

AU - Trivelli, Xavier

AU - Venkatesan, Gopalakrishnan

AU - Bezzubov, Stanislav I.

AU - Kajjout, Mohammed

AU - Gushchin, Artem L.

AU - Hanif, Muhammad

AU - Poizat, Olivier

AU - Vezin, Hervé

AU - Rolando, Christian

PY - 2020/4/6

Y1 - 2020/4/6

N2 - The in-gel detection of proteins for various proteomic experiments is commonly done with the fluorescent RuII tris(bathophenanthroline disulfonate) complex (Ru(BPS)3), which is more cost-effective compared to commercial Ru-based formulations but requires tedious procedures for its preparation and strongly acidic staining conditions. Herein, we report the synthesis and characterization of heteroleptic RuII complexes Ru(BPS)2(BP) and Ru(BPS)(BP)2 containing bathophenanthroline (BP) and bathophenanthroline disulfonate disodium salt (BPS) in comparison with Ru(BPS)3. It was shown by fluorescent and UV-vis measurements that novel RuII complexes were excitable in both UV and visible light, close to emission bands of classical lasers, which is important for successful in-gel protein detection. Novel fluorescent dyes demonstrated improved protein detection in comparison with commercially available SYPRO Ruby staining solution. In addition, unlike commonly used staining protocols, staining with Ru(BPS)(BP)2 can be performed at nearly neutral pH, thereby reducing artificial post-translational modifications (PTMs).

AB - The in-gel detection of proteins for various proteomic experiments is commonly done with the fluorescent RuII tris(bathophenanthroline disulfonate) complex (Ru(BPS)3), which is more cost-effective compared to commercial Ru-based formulations but requires tedious procedures for its preparation and strongly acidic staining conditions. Herein, we report the synthesis and characterization of heteroleptic RuII complexes Ru(BPS)2(BP) and Ru(BPS)(BP)2 containing bathophenanthroline (BP) and bathophenanthroline disulfonate disodium salt (BPS) in comparison with Ru(BPS)3. It was shown by fluorescent and UV-vis measurements that novel RuII complexes were excitable in both UV and visible light, close to emission bands of classical lasers, which is important for successful in-gel protein detection. Novel fluorescent dyes demonstrated improved protein detection in comparison with commercially available SYPRO Ruby staining solution. In addition, unlike commonly used staining protocols, staining with Ru(BPS)(BP)2 can be performed at nearly neutral pH, thereby reducing artificial post-translational modifications (PTMs).

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UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85082314450&origin=recordpage

U2 - 10.1021/acs.inorgchem.9b03679

DO - 10.1021/acs.inorgchem.9b03679

M3 - RGC 21 - Publication in refereed journal

C2 - 32181663

SN - 0020-1669

VL - 59

SP - 4527

EP - 4535

JO - Inorganic Chemistry

JF - Inorganic Chemistry

IS - 7

ER -

Heteroleptic Ruthenium(II) Complexes with Bathophenanthroline and Bathophenanthroline Disulfonate Disodium Salt as Fluorescent Dyes for In-Gel Protein Staining

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