Harnessing oleaginous protist Schizochytrium for docosahexaenoic acid: Current technologies in sustainable production and food applications

Docosahexaenoic acid (DHA) exerts versatile roles in nutrition supplementation and numerous health disorders prevention. Global consumption demand for DHA has also been consistently increasing with enhanced health awareness. Oleaginous marine protist Schizochytrium is praised as a potential DHA source due to short growth cycle, convenient artificial culture, harmless to the human body, and easy manipulation of the DHA synthesis pathway. However, factors including strain performances, fermentation parameters, product harvest and extraction strategies, safety and stability maintenance, and also application limitations in health and functional properties affect the widespread adoption of Schizochytrium DHA products. This review provides a comprehensive summary of the current biotechnologies used for tackling factors affecting the Schizochytrium DHA production, with special focuses on Schizochytrium strain improvement technologies, fermentation optimization projects, DHA oil extraction strategies, safety evaluations and stability maintenance schemes, and DHA product application approaches in foods. Inspired by systematic literature investigations and recent advances, suggestive observations composed of improving strain with multiple breeding technologies, considering artificial intelligence and machine learning to optimize the fermentative process, introducing nanoparticles packing technology to improve oxidation stability of DHA products, covering up DHA odor defect with characteristic flavor foods, and employing synthetic biology to construct the structured lipids with DHA to exploit potential functions are formed. This review will give a guideline for exploring more Schizochytrium DHA and propelling the application development in food and health. © 2025 The Authors.