Gravitational sedimentation-based approach for ultra-simple and flexible cell patterning coculture on microfluidic device

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

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Detail(s)

Original languageEnglish
Article number035005
Number of pages12
Journal / PublicationBiofabrication
Volume12
Issue number3
Online published27 Apr 2020
Publication statusPublished - Jul 2020

Abstract

Combining patterning coculture technique with microfluidics enables the reconstruction of complex in-vivo system to facilitate in-vitro studies on cell-cell and cell-environment interactions. However, simple and versatile approaches for patterning coculture of cells on microfluidic platforms remain lacking. In this study, a novel gravitational sedimentation-based approach is presented to achieve ultra-simple and flexible cell patterning coculture on a microfluidic platform, where multiple cell types can be patterned simultaneously to form a well-organized cell coculture. In contrast to other approaches, the proposed approach allows the rapid patterning of multiple cell types in microfluidic channels without the use of sheath flow and a prepatterned functional surface. This feature greatly simplifies the experimental setup, operation, and chip fabrication. Moreover, cell patterning can be adjusted by simply modifying the cell-loading tubing direction, thereby enabling great flexibility for the construction of different cell patterns without complicating the chip design and flow control. A series of physical and biological experiments are conducted to validate the proposed approach. This research paves a new way for building physiologically realistic in-vitro coculture models on microfluidic platforms for various applications, such as cell-cell interaction and drug screening.

Research Area(s)

  • gravitational sedimentation, microfluidics, cell patterning coculture

Citation Format(s)

Gravitational sedimentation-based approach for ultra-simple and flexible cell patterning coculture on microfluidic device. / Fan, Lei; Luo, Tao; Guan, Zhangyan; Chow, Yu Ting; Chen, Shuxun; Wei, Tanyong; Shakoor, Adnan; Lam, Raymond H W; Sun, Dong.

In: Biofabrication, Vol. 12, No. 3, 035005, 07.2020.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal