Granular Sludge Coupling Nitrate/Nitrite Dependent Anaerobic Methane Oxidation with Anammox : From Proof-of-Concept to High Rate Nitrogen Removal

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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Author(s)

  • Sheng-Qiang Fan
  • Guo-Jun Xie
  • Yang Lu
  • Bing-Feng Liu
  • De-Feng Xing
  • Hong-Jun Han
  • Nan-Qi Ren

Detail(s)

Original languageEnglish
Pages (from-to)297–305
Journal / PublicationEnvironmental Science and Technology
Volume54
Issue number1
Online published2 Dec 2019
Publication statusPublished - 7 Jan 2020
Externally publishedYes

Abstract

This work developed a novel Membrane Granular Sludge Reactor (MGSR) equipped with a gas permeable membrane module for efficient methane delivery to cultivate nitrate/nitrite dependent anaerobic methane oxidation (n-DAMO) microorganisms in granular sludge. As proof of concept, the MGSR was fed with synthetic wastewater containing nitrate and ammonium to facilitate the growth of n-DAMO microorganisms. The granular sludge of n-DAMO and Anammox was gradually developed and achieved a nitrogen removal rate of 1.08 g NO3--N L-1 d-1 and 0.81 g NH4+-N L-1 d-1. Finally, enriched granular sludge was successfully applied for nitrogen removal from the synthetic partial nitritation effluent. The combined dominance of n-DAMO archaea, Anammox bacteria, and n-DAMO bacteria in the microbial community was confirmed by 16S rRNA amplicon sequencing. Fluorescence in situ hybridization revealed that a layered structure was formed in the granular sludge with Anammox bacteria in the outer layer and n-DAMO microorganisms in the inner layer when granules were fed with nitrite and ammonium. The high performance of nitrogen removal (16.53 kg N m-3 d-1) with satisfactory effluent quality (∼8 mg N L-1) and excellent biomass retention capacity (43 g VSS L-1) make the MGSR promising for the practical application of n-DAMO and Anammox in wastewater treatment. © 2019 American Chemical Society.

Citation Format(s)

Granular Sludge Coupling Nitrate/Nitrite Dependent Anaerobic Methane Oxidation with Anammox: From Proof-of-Concept to High Rate Nitrogen Removal. / Fan, Sheng-Qiang; Xie, Guo-Jun; Lu, Yang et al.
In: Environmental Science and Technology, Vol. 54, No. 1, 07.01.2020, p. 297–305.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review