G-quadruplex formation and sequence effect on the assembly of G-rich oligonucleotides induced by Pb2+ ions

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Wei Liu
  • Bin Zheng
  • Sheng Cheng
  • Yan Fu
  • Wei Li
  • Haojun Liang

Detail(s)

Original languageEnglish
Pages (from-to)7017-7023
Journal / PublicationSoft Matter
Volume8
Issue number26
Publication statusPublished - 14 Jul 2012

Abstract

The lead-induced folding into G-quadruplex structures for three guanine-rich oligonucleotides, two model human telomeric oligomers (HTG/T2) and thrombin-binding aptamer (TBA), was characterized by equilibrium titrations, rapid mixing techniques and stopped-flow kinetics. The analysis of optical titration data reveals that the saturated Pb2+-DNA binding stoichiometries are 1:1, 2:1 and 3:1 for TBA, HTG and T2, respectively. Thermal denaturation experiments were performed to determine the structural stability. The overall results are consistent with the higher stability of T2 that contains four G-quartets, as opposed to HTG and TBA that have only three and two quartets, respectively. We also present kinetic studies of the formation of G-quadruplexes of G-rich DNA induced by Pb2+ ions. The binding of Pb2+ ions to G-DNA is a complex multiple pathway process, which is affected by the sequence of the G-DNA. The studies show that the connecting loops of the DNA play an important role in modulating the structures. © The Royal Society of Chemistry.

Citation Format(s)

G-quadruplex formation and sequence effect on the assembly of G-rich oligonucleotides induced by Pb2+ ions. / Liu, Wei; Zheng, Bin; Cheng, Sheng; Fu, Yan; Li, Wei; Lau, Tai-Chu; Liang, Haojun.

In: Soft Matter, Vol. 8, No. 26, 14.07.2012, p. 7017-7023.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review