G-protein-coupled estrogen receptor 1 is involved in brain development during zebrafish (Danio rerio) embryogenesis

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

25 Scopus Citations
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Author(s)

  • Yanan Shi
  • Xiaochun Liu
  • Pei Zhu
  • Jianzhen Li
  • Kathy W.Y. Sham
  • Shuisheng Li
  • Yong Zhang
  • Christopher H.K. Cheng
  • Haoran Lin

Detail(s)

Original languageEnglish
Pages (from-to)21-27
Journal / PublicationBiochemical and Biophysical Research Communications
Volume435
Issue number1
Publication statusPublished - 24 May 2013

Abstract

G-protein-coupled estrogen receptor 1 (Gper, formerly known as GPR30) is found to be a trophic and protective factor in mediating action of estrogen in adult brain, while its role in developing brain remains to be elucidated. Here we present the expression pattern of Gper and its functions during embryogenesis in zebrafish. Both the mRNA and protein of Gper were detected throughout embryogenesis. Whole mount in situ hybridization (WISH) revealed a wide distribution of gper mRNAs in various regions of the developing brain. Gper knockdown by specific morpholinos resulted in growth retardation in embryos and morphological defects in the developing brain. In addition, induced apoptosis, decreased proliferation of the brain cells and maldevelopment of sensory and motor neurons were also found in the morphants. Our results provide novel insights into Gper functions in the developing brain, revealing that Gper can maintain the survival of the brain cells, and formation and/or differentiation of the sensory and motor neurons. © 2013 Elsevier Inc.

Research Area(s)

  • Gper, Morpholino, Zebrafish

Citation Format(s)

G-protein-coupled estrogen receptor 1 is involved in brain development during zebrafish (Danio rerio) embryogenesis. / Shi, Yanan; Liu, Xiaochun; Zhu, Pei; Li, Jianzhen; Sham, Kathy W.Y.; Cheng, Shuk Han; Li, Shuisheng; Zhang, Yong; Cheng, Christopher H.K.; Lin, Haoran.

In: Biochemical and Biophysical Research Communications, Vol. 435, No. 1, 24.05.2013, p. 21-27.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review