TY - GEN
T1 - Gold nanoparticle-assisted single base-pair mismatch discrimination on a microfluidic microarray device
AU - Wang, Lin
AU - Li, Paul C.H.
N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].
PY - 2009
Y1 - 2009
N2 - A simple gold nanoparticle (GNP)-based DNA analysis on a microfluidic device is presented. With the aid of GNPs, we have successfully discriminated between two DNAs (derived from closely related fungal pathogens, Botrytis cinerea and Botrytis squamosa) with one-base-pair difference. Microfluidic chips avoid the use of large sample volumes and only a small amount of oligonucelotides (8-fmol) or PCR products (3-ng) was needed. The whole procedure is finished at room temperature in an hour, and no high stringency method performed at elevated temperature is required. © 2009 CBMS.
AB - A simple gold nanoparticle (GNP)-based DNA analysis on a microfluidic device is presented. With the aid of GNPs, we have successfully discriminated between two DNAs (derived from closely related fungal pathogens, Botrytis cinerea and Botrytis squamosa) with one-base-pair difference. Microfluidic chips avoid the use of large sample volumes and only a small amount of oligonucelotides (8-fmol) or PCR products (3-ng) was needed. The whole procedure is finished at room temperature in an hour, and no high stringency method performed at elevated temperature is required. © 2009 CBMS.
KW - Fungal pathogen DNA
KW - Gold nanoparticles
KW - Microfluidic DNA microarray
KW - Single base-pair discrimination
KW - Fungal pathogen DNA
KW - Gold nanoparticles
KW - Microfluidic DNA microarray
KW - Single base-pair discrimination
KW - Fungal pathogen DNA
KW - Gold nanoparticles
KW - Microfluidic DNA microarray
KW - Single base-pair discrimination
UR - https://www.scopus.com/pages/publications/84901747246
UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-84901747246&origin=recordpage
M3 - RGC 32 - Refereed conference paper (with host publication)
SN - 9780979806421
T3 - Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 1127
EP - 1129
BT - Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
Y2 - 1 November 2009 through 5 November 2009
ER -