Glucosamine-modified polyethylene glycol hydrogel-mediated chondrogenic differentiation of human mesenchymal stem cells

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

31 Scopus Citations
View graph of relations

Author(s)

  • Hang Yao
  • Jingchen Xue
  • Qunfang Wang
  • Renjian Xie
  • Weichang Li
  • Sa Liu
  • Jinglei Cai
  • Dajiang Qin
  • Li Ren

Detail(s)

Original languageEnglish
Pages (from-to)661-670
Journal / PublicationMaterials Science and Engineering C
Volume79
Online published11 May 2017
Publication statusPublished - 1 Oct 2017
Externally publishedYes

Abstract

Glucosamine (GA) is an important cartilage matrix precursor for the glycosaminoglycan biochemical synthesis, and has positive effects on cartilage regeneration, particularly in osteoarthritis therapy. However, it has not been used as a bioactive group in scaffolds for cartilage repair widely. In this study, we synthesized modified polyethylene glycol (PEG) hydrogel with glucosamine and then encapsulated human bone mesenchymal stem cells (hBMSCs) in the hydrogel to induce the differentiation of hBMSCs into chondrocytes in three-dimensional culture. The GA-modified PEG hydrogels promoted the chondrogenesis of hBMSCs, particularly in the concentration of 5 mM and 10 mM. The subcutaneous transplantation of 10 mM GA-modified hydrogels with hBMSCs formed cartilage-like blocks in vivo for 8 weeks. Importantly, with glucosamine increase, the modified hydrogels down-regulated the fibrosis and hypertrophic cartilage markers in protein level. Therefore, glucosamine modified PEG hydrogels facilitated the chondrogenesis of hBMSCs, which might represent a new method for cartilage repair using a tissue-engineering approach.

Research Area(s)

  • Bone mesenchymal stem cells, Chondrogenesis, Glucosamine, Polyethylene glycol hydrogel

Citation Format(s)

Glucosamine-modified polyethylene glycol hydrogel-mediated chondrogenic differentiation of human mesenchymal stem cells. / Yao, Hang; Xue, Jingchen; Wang, Qunfang et al.
In: Materials Science and Engineering C, Vol. 79, 01.10.2017, p. 661-670.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review