Glucocorticoid receptor is involved in the differential expression of hepatic 3β-hydroxysteroid dehydrogenase between barrows and boars at finishing stage

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

View graph of relations

Author(s)

  • Xian Li
  • Rihua Cong
  • Wen Yao
  • Yimin Jia
  • Zhiyuan Sun
  • Xi Li
  • Ruqian Zhao

Detail(s)

Original languageEnglish
Pages (from-to)158-166
Number of pages9
Journal / PublicationAnimal Science Journal
Volume89
Issue number1
Online published6 Sep 2017
Publication statusPublished - Jan 2018
Externally publishedYes

Abstract

The enzyme 3β-hydroxysteroid dehydrogenase (3β-HSD) plays an important role in androstenone metabolism in pig liver, and its defective expression is related to the development of boar taint. Early age castration is a common practice in many countries to avoid boar taint, yet whether and how castration affects porcine hepatic 3β-HSD expression are still poorly understood. In this study, we aimed to compare the expression of 3β-HSD between intact (boars) and castrated (barrows) male pigs, and to explore the potential factors regulating 3β-HSD transcription. Compared to barrows, boars showed worse carcass quality. Boars had significantly higher levels of serum androstenone (P < 0.01), testosterone (P < 0.01) and hepatic cortisol (P < 0.05), which were contrary to significantly lower expression of 3β-HSD messenger RNA (P < 0.01) and protein (P < 0.01) in the liver. Significant differences were detected for the hepatic expression of androgen receptor (AR) and CCAAT/enhancer binding protein β (C/EBPβ). Chromatin immunoprecipitation (ChIP) assay demonstrated reduced histone H3 acetylation (< 0.05) but increased glucocorticoid receptor (GR) binding to 3β-HSD gene promoter in boars (P < 0.05). These results indicate that GR binding to 3β-HSD promoter is involved in the differential hepatic 3β-HSD expression between boars and barrows.

Research Area(s)

  • 3β-HSD, androgen receptor, glucocorticoid receptor, liver, pig

Citation Format(s)

Glucocorticoid receptor is involved in the differential expression of hepatic 3β-hydroxysteroid dehydrogenase between barrows and boars at finishing stage. / Li, Xian; Cong, Rihua; Yao, Wen; Jia, Yimin; Li, Runsheng; Sun, Zhiyuan; Li, Xi; Zhao, Ruqian.

In: Animal Science Journal, Vol. 89, No. 1, 01.2018, p. 158-166.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review