Global cytokine/chemokine profile identifies potential progression prediction indicators in hand-foot-and-mouth disease patients with Enterovirus A71 infections

Yaqing He; Zhuoying Feng; Wei Wang; Ying Chen; Jinquan Cheng; Jun Meng; Hong Yang; Yujie Wang; Xiangjie Yao; Qianjin Feng; Long Chen; Hailong Zhang; Maggie H.T. Wang; Benny C.Y. Zee; Xin Wang; Ming Liang HE

doi:10.1016/j.cyto.2019.154765

Global cytokine/chemokine profile identifies potential progression prediction indicators in hand-foot-and-mouth disease patients with Enterovirus A71 infections

Yaqing He, Zhuoying Feng, Wei Wang, Ying Chen, Jinquan Cheng, Jun Meng, Hong Yang, Yujie Wang, Xiangjie Yao, Qianjin Feng, Long Chen, Hailong Zhang, Maggie H.T. Wang, Benny C.Y. Zee, Xin Wang, Ming Liang HE^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

15 Citations (Scopus)

Abstract

Objective: New clinical indicators are urgently needed for predicting the progression and complications of hand-foot-and-mouth disease (HFMD) caused by EV-A71 infections.
Materials and Methods: Serum specimens from 132 EV-A71 HFMD patients and 73 health children were collected during 2012 to 2014 in Shenzhen, China. The specific cytokines/chemokines were detected with a 274-human cytokine antibody array, followed by a 38-inflammation cytokine array, and further validated by ELISA.
Results: Cytokines varied in different severity of EV-A71 HFMD patients. The ROC curve analysis revealed 6 serum cytokines with high sensitivity and specificity in predicting the disease progression. Eotaxin, IL-8 and IP-10 have showed high AUC values (0.90 - 0.95) for discrimination between the health controls and the patient group. The three cytokines showed high sensitivity (80% - 91%) and specificity (88% - 95%). MMP-8 had a high sensitivity and specificity to predict mild HFMD (100%, 100%). IL-1b and leptin discriminated the severe/critical group from the mild group (79% and 69% in sensitivity, 73% and 63% in specificity).
Conclusions: Eotaxin, IP-10 and IL-8 could be potential indicators for predicting HFMD progression with EV-A71 infection. MMP-8 is a specific indicator for mild infection, while IL-1b and leptin display potential for predicting the severity and criticality.

Original language	English
Article number	154765
Journal	Cytokine
Volume	123
Online published	27 Jun 2019
DOIs	https://doi.org/10.1016/j.cyto.2019.154765
Publication status	Published - Nov 2019

Research Keywords

Cytokine/chemokine array
EV-A71
Hand-foot-and-mouth disease
Indicator
Inflammation profile

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RGC-funded

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He, Y., Feng, Z., Wang, W., Chen, Y., Cheng, J., Meng, J., Yang, H., Wang, Y., Yao, X., Feng, Q., Chen, L., Zhang, H., Wang, M. H. T., Zee, B. C. Y., Wang, X., & HE, M. L. (2019). Global cytokine/chemokine profile identifies potential progression prediction indicators in hand-foot-and-mouth disease patients with Enterovirus A71 infections. Cytokine, 123, Article 154765. https://doi.org/10.1016/j.cyto.2019.154765

@article{b080005e88eb49e79f586523da8ab2f6,

title = "Global cytokine/chemokine profile identifies potential progression prediction indicators in hand-foot-and-mouth disease patients with Enterovirus A71 infections",

abstract = "Objective: New clinical indicators are urgently needed for predicting the progression and complications of hand-foot-and-mouth disease (HFMD) caused by EV-A71 infections. Materials and Methods: Serum specimens from 132 EV-A71 HFMD patients and 73 health children were collected during 2012 to 2014 in Shenzhen, China. The specific cytokines/chemokines were detected with a 274-human cytokine antibody array, followed by a 38-inflammation cytokine array, and further validated by ELISA.Results: Cytokines varied in different severity of EV-A71 HFMD patients. The ROC curve analysis revealed 6 serum cytokines with high sensitivity and specificity in predicting the disease progression. Eotaxin, IL-8 and IP-10 have showed high AUC values (0.90 - 0.95) for discrimination between the health controls and the patient group. The three cytokines showed high sensitivity (80% - 91%) and specificity (88% - 95%). MMP-8 had a high sensitivity and specificity to predict mild HFMD (100%, 100%). IL-1b and leptin discriminated the severe/critical group from the mild group (79% and 69% in sensitivity, 73% and 63% in specificity). Conclusions: Eotaxin, IP-10 and IL-8 could be potential indicators for predicting HFMD progression with EV-A71 infection. MMP-8 is a specific indicator for mild infection, while IL-1b and leptin display potential for predicting the severity and criticality.",

keywords = "Cytokine/chemokine array, EV-A71, Hand-foot-and-mouth disease, Indicator, Inflammation profile",

author = "Yaqing He and Zhuoying Feng and Wei Wang and Ying Chen and Jinquan Cheng and Jun Meng and Hong Yang and Yujie Wang and Xiangjie Yao and Qianjin Feng and Long Chen and Hailong Zhang and Wang, {Maggie H.T.} and Zee, {Benny C.Y.} and Xin Wang and HE, {Ming Liang}",

year = "2019",

month = nov,

doi = "10.1016/j.cyto.2019.154765",

language = "English",

volume = "123",

journal = "Cytokine",

issn = "1043-4666",

publisher = "Academic Press",

}

He, Y, Feng, Z , Wang, W, Chen, Y, Cheng, J, Meng, J, Yang, H, Wang, Y, Yao, X, Feng, Q, Chen, L, Zhang, H, Wang, MHT, Zee, BCY, Wang, X & HE, ML 2019, 'Global cytokine/chemokine profile identifies potential progression prediction indicators in hand-foot-and-mouth disease patients with Enterovirus A71 infections', Cytokine, vol. 123, 154765. https://doi.org/10.1016/j.cyto.2019.154765

TY - JOUR

T1 - Global cytokine/chemokine profile identifies potential progression prediction indicators in hand-foot-and-mouth disease patients with Enterovirus A71 infections

AU - He, Yaqing

AU - Feng, Zhuoying

AU - Wang, Wei

AU - Chen, Ying

AU - Cheng, Jinquan

AU - Meng, Jun

AU - Yang, Hong

AU - Wang, Yujie

AU - Yao, Xiangjie

AU - Feng, Qianjin

AU - Chen, Long

AU - Zhang, Hailong

AU - Wang, Maggie H.T.

AU - Zee, Benny C.Y.

AU - Wang, Xin

AU - HE, Ming Liang

PY - 2019/11

Y1 - 2019/11

N2 - Objective: New clinical indicators are urgently needed for predicting the progression and complications of hand-foot-and-mouth disease (HFMD) caused by EV-A71 infections. Materials and Methods: Serum specimens from 132 EV-A71 HFMD patients and 73 health children were collected during 2012 to 2014 in Shenzhen, China. The specific cytokines/chemokines were detected with a 274-human cytokine antibody array, followed by a 38-inflammation cytokine array, and further validated by ELISA.Results: Cytokines varied in different severity of EV-A71 HFMD patients. The ROC curve analysis revealed 6 serum cytokines with high sensitivity and specificity in predicting the disease progression. Eotaxin, IL-8 and IP-10 have showed high AUC values (0.90 - 0.95) for discrimination between the health controls and the patient group. The three cytokines showed high sensitivity (80% - 91%) and specificity (88% - 95%). MMP-8 had a high sensitivity and specificity to predict mild HFMD (100%, 100%). IL-1b and leptin discriminated the severe/critical group from the mild group (79% and 69% in sensitivity, 73% and 63% in specificity). Conclusions: Eotaxin, IP-10 and IL-8 could be potential indicators for predicting HFMD progression with EV-A71 infection. MMP-8 is a specific indicator for mild infection, while IL-1b and leptin display potential for predicting the severity and criticality.

AB - Objective: New clinical indicators are urgently needed for predicting the progression and complications of hand-foot-and-mouth disease (HFMD) caused by EV-A71 infections. Materials and Methods: Serum specimens from 132 EV-A71 HFMD patients and 73 health children were collected during 2012 to 2014 in Shenzhen, China. The specific cytokines/chemokines were detected with a 274-human cytokine antibody array, followed by a 38-inflammation cytokine array, and further validated by ELISA.Results: Cytokines varied in different severity of EV-A71 HFMD patients. The ROC curve analysis revealed 6 serum cytokines with high sensitivity and specificity in predicting the disease progression. Eotaxin, IL-8 and IP-10 have showed high AUC values (0.90 - 0.95) for discrimination between the health controls and the patient group. The three cytokines showed high sensitivity (80% - 91%) and specificity (88% - 95%). MMP-8 had a high sensitivity and specificity to predict mild HFMD (100%, 100%). IL-1b and leptin discriminated the severe/critical group from the mild group (79% and 69% in sensitivity, 73% and 63% in specificity). Conclusions: Eotaxin, IP-10 and IL-8 could be potential indicators for predicting HFMD progression with EV-A71 infection. MMP-8 is a specific indicator for mild infection, while IL-1b and leptin display potential for predicting the severity and criticality.

KW - Cytokine/chemokine array

KW - EV-A71

KW - Hand-foot-and-mouth disease

KW - Indicator

KW - Inflammation profile

UR - http://www.scopus.com/inward/record.url?scp=85067929762&partnerID=8YFLogxK

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85067929762&origin=recordpage

U2 - 10.1016/j.cyto.2019.154765

DO - 10.1016/j.cyto.2019.154765

M3 - RGC 21 - Publication in refereed journal

SN - 1043-4666

VL - 123

JO - Cytokine

JF - Cytokine

M1 - 154765

ER -

Global cytokine/chemokine profile identifies potential progression prediction indicators in hand-foot-and-mouth disease patients with Enterovirus A71 infections

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GRF: The Molecular Mechanism of PIM1 Response to Facilitate Human Enterovirus 71 Infection

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Global cytokine/chemokine profile identifies potential progression prediction indicators in hand-foot-and-mouth disease patients with Enterovirus A71 infections

Abstract

Research Keywords

RGC Funding Information

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GRF: The Molecular Mechanism of PIM1 Response to Facilitate Human Enterovirus 71 Infection

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